Transcriptomics

Dataset Information

4

Expression data on human MO-to-DC and MO-to-MAC differentiation


ABSTRACT: Comparison of the RNA expression profiles of CD14+ monocytes from human peripheral blood with derived dendritic cells (DCs) and macrophages (MACs) obtained by exposure with GM-CSF/IL-4 and GM-CSF, respectively, and with mature DCs and MACs after lipopolysaccharide (LPS) exposure The expression profiles of RNA of human CD14+ monocytes were compared with derived immature dendritic cells (iDCs) and macrophages (iMACs) following GM-CSF/IL-4 and GM-CSF incubation, and then activation/maturation with lypopolysaccharyde (LPS) using the Affymetrix PrimeView Human Gene Expression array (Affymetrix, Santa Clara, CA). This platform allows the interrogation of >36,000 transcrits and variants per sample. The samples were hybridized in the array following the manufacturer’s instructions. Total RNA isolated by standard procedures from CD14+ cells (total monocytes, MOs) corresponding to three sets of samples of monocytes (MOs), derived immature DCs and MACs (iDCS and iMACS) and activated/mature DCs and MACs following incubation with LPS (mDCS and mMACs)

ORGANISM(S): Homo sapiens  

SUBMITTER: Radhakrishnan Sabarinathan   Ana Luque  Javier Rodríguez-Ubreva  Jose M Urquiza  Damiana Álvarez-Errico  Esteban Ballestar  Lorenzo de la Rica  Carlos Company  Josep M Aran  Biola M Javierre  Manel Esteller  Roser Vento-Tormo 

PROVIDER: E-GEOD-75938 | ArrayExpress | 2015-12-12

SECONDARY ACCESSION(S): GSE75938PRJNA305747

REPOSITORIES: GEO, ArrayExpress

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