Transcriptomics

Dataset Information

297

Desiccation Tolerance of Arabidopsis thaliana seeds


ABSTRACT: Desiccation tolerance (DT) allowed seed plants to conquer ecosystems with long periods of limited water availability. This adaptive features allows seeds to remain dried for very long times without losing their ability to germinate. There is little information about all the signaling components required to achieve DT and on how transcription factors (TFs) modulate global DT processes. We performed RNA-seq experiment and carbohydrates profiles of lec1, lec2, fus3 and abi3, as well as their corresponding wild types, at three stages of seed development 15, 17 and 21 DAF (day after open flower) belonging to the seed desiccation period. A complex experimental design approach and regulatory networks prediction were used to identify differentially expressed genes specifically involved in DT process. In order to identify mechanisms involved in the acquisition of DT during seed development, we designed a comparative transcriptomic analysis between the seed desiccation intolerant (DI) mutants lec1-1, abi3-5 and fus3-3, the desiccation tolerant mutant lec2-1 and the desiccation tolerant weak allele of abi3 (abi3-1) with their respective wild type controls. This analysis should allow to identify genes that are differentially expressed in the desiccation intolerant mutants respect to tolerant mutants and WT controls.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Luis H Estrella   Sandra I Gonzalez-Morales  Sandra Isabel Gonzalez-Morales 

PROVIDER: E-GEOD-76015 | ArrayExpress | 2016-09-06

SECONDARY ACCESSION(S): GSE76015SRP067396PRJNA306014

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana.

González-Morales Sandra Isabel SI   Chávez-Montes Ricardo A RA   Hayano-Kanashiro Corina C   Alejo-Jacuinde Gerardo G   Rico-Cambron Thelma Y TY   de Folter Stefan S   Herrera-Estrella Luis L  

Proceedings of the National Academy of Sciences of the United States of America 20160822 35


Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolu  ...[more]

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