Transcriptomics

Dataset Information

4

Gene expression profiling of endothelial cells derived from Foxo1(+/+) and Foxo1(-/-) ES cells in response to VEGF


ABSTRACT: VEGF induces elongation of endothelial cells (ECs) that are derived from wild-type (Foxo1(+/+)) ES cells. VEGF-induced EC elongation is an important process of angiogenesis. ECs derived from Foxo1(-/-) ES cells fail to elongate in response to VEGF. Gene expression profiling of wild-type and Foxo1(-/-) ECs in the presence or absence of VEGF stimulation identifies responsible genes that regulate EC elongation. One wild-type (Foxo1(+/+)) ES cell clone and one Foxo1(-/-) ES cell clone were used. ES cells were cultured on OP9 stromal cell layer in the presence or absence of VEGF (10 ng/ml). After 6.5 days, VE-cadherin+ CD31+ ECs were isolated by FACS and used for total RNA extraction. Three independent experiments were performed for each condition.

ORGANISM(S): Mus musculus  

SUBMITTER: Minetaro Ogawa   Satoshi Kondo  Kiyomi Tsuji-Tamura 

PROVIDER: E-GEOD-76366 | ArrayExpress | 2016-02-16

SECONDARY ACCESSION(S): GSE76366PRJNA307103

REPOSITORIES: GEO, ArrayExpress

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