Transcriptomics

Dataset Information

62

Single-cell RNA-seq of Ecad positive PyMT bulk tumor cells


ABSTRACT: In this study we studied the presence of tumor cells that underwent epithelial-to-mesenchymal transition within polyoma middle T antigen (PyMT) breast tumors. For this we dissociated tumors and isolated Ecad positive tumor cells by FACS sorting. We confirmed that PyMT tumors contain a small set of tumor cells that have undergone EMT in the primary tumor and that E-cadherin can be used as a marker on single cell level for mesenchymal status in this model. We isolated primary tumors from mice, dissociated the tumors and FACS-sorted for single Ecad positive tumor cells, after this we performed single cell sequencing of the cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Jean-Charles Boisset  Alexander van Oudenaarden   Jacco van Rheenen   Danielle Seinstra   Lennart Kester    

PROVIDER: E-GEOD-77107 | ArrayExpress | 2016-03-17

SECONDARY ACCESSION(S): SRP068739GSE77107PRJNA309444

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


Forced overexpression and/or downregulation of proteins regulating epithelial-to-mesenchymal transition (EMT) has been reported to alter metastasis by changing migration and stem cell capacity of tumor cells. However, these manipulations artificially keep cells in fixed states, while in vivo cells may adapt transient and reversible states. Here, we have tested the existence and role of epithelial-mesenchymal plasticity in metastasis of mammary tumors without artificially modifying EMT regulators  ...[more]

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