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DNA methylation in the arcuate nucleus of heifers under two nutritional schedules

ABSTRACT: DNA methylation was assessed in genomic DNA obtained from the arcuate nucleus of heifers fed to gain body weight at high (HG, n = 4) and low (LG, n = 4) rates from 4.5 to 8.5 mo of age. A methyl-CpG binding domain-based (MBD) protein assay was performed to capture fragments of methylated DNA (methylated-enriched DNA). Input (total) and methylated-enriched DNA were labeled with two different dyes and co-hybridized to a custom-designed oligonucleotide array targeted to genes associated with nutritional inputs and the control of puberty. The ratio of the log2 (enriched/input) of the normalized intensities, were determined. Data was analyzed comparing values of HG versus LG heifers. Two nutritional schedules: HG (n=4 heifers) and LG (n=4 heifers); one array per heifer; methylated enriched DNA (enriched) and total DNA (input) co-hybridyzed into each array Methylated-enriched DNA obtained from a methyl-CpG binding domain-based (MBD) protein assay

ORGANISM(S): Bos taurus

SUBMITTER: Rodolfo C Cardoso Bruna Rios Coelho Alves Ryan Doan Scott V Dindot Marcel Amstalden Gary L Williams Bruna R Alves

PROVIDER: E-GEOD-77782 | ArrayExpress | 2016-02-11

SECONDARY ACCESSION(S): GSE77782PRJNA311548

REPOSITORIES: GEO, ArrayExpress

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