Transcriptomics

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Transcription profiling by high throughput sequencing of four potato reference cultivars with differential drought tolerance in response to drought stress


ABSTRACT: Systems responses of mature leaves from 4 reference cultivars of a larger collection of European potato cultivars (Solanum tuberosum L.) are investigated by metabolome profiling and RNA-Sequencing. The chosen reference cultivars, Milva, Alegria, Desiree, and Saturna, vary in ascending order in regard to drought tolerance. Systems analyses are based on 3 independent field trials and 3 paralleled greenhouse trials. Robust responses across all cultivars and conditions to natural seasonal drought stress comprise proline, raffinose, galactinol, arabitol, arabinonic acid, chlorogenic acid, and 102 transcripts which consist to a high proportion of heat shock proteins and genes with signaling or regulatory functions, such as a homolog of abscisic acid receptor PYL4. Constitutive differences of the tolerant cultivars, Desiree and Saturna, compared to the sensitive cultivars include arbutin (hydroquinone-beta-D-glucopyranoside), octopamine (p-hydroxyphenylethanolamine), ribitol and 248 differential transcripts. Many of these transcripts are disease related, receptor kinases, or regulatory genes, for example a homolog of the Arabidopsis FOUR LIPS MYB-regulator of stomatal cell proliferation. Functional enrichment analyses imply that heat stress is a major acclimation component of potato leaves to agronomical relevant drought stress. Enhanced leaf heat stress is a result of drought caused by loss of transpiration cooling. This effect and CO2-limitation are the main dilemmas of drought- or ABA-induced stomatal closure. Constitutive differences between tolerant and sensitive cultivars indicate partially synergistic interactions of drought and biotic stress responses. We suggest that drought tolerance of the potato reference cultivars may be caused by general resistance mechanisms which are part of previously selected pathogen tolerance. Transcriptome profiling by RNA-sequencing of 48 leaf samples from 4 potato cultivars grown under control or drought stress conditions in 6 independent experiments

ORGANISM(S): Solanum tuberosum  

SUBMITTER: Axel Fischer   Alexander Erban  Ellen Zuther  Joachim Kopka  Heike Sprenger  Karin Kohl  Christina Kurowsky  Katharina Rudack  Renate Horn  Sylvia Seddig  Dirk K Hincha  Dirk Walther 

PROVIDER: E-GEOD-77826 | ArrayExpress | 2016-07-06

SECONDARY ACCESSION(S): GSE77826SRP069961PRJNA311702

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The drought response of potato reference cultivars with contrasting tolerance.

Sprenger Heike H   Kurowsky Christina C   Horn Renate R   Erban Alexander A   Seddig Sylvia S   Rudack Katharina K   Fischer Axel A   Walther Dirk D   Zuther Ellen E   Köhl Karin K   Hincha Dirk K DK   Kopka Joachim J  

Plant, cell & environment 20160906 11


Systems responses to drought stress of four potato reference cultivars with differential drought tolerance (Solanum tuberosum L.) were investigated by metabolome profiling and RNA sequencing. Systems analysis was based on independent field and greenhouse trials. Robust differential drought responses across all cultivars under both conditions comprised changes of proline, raffinose, galactinol, arabitol, arabinonic acid, chlorogenic acid and 102 transcript levels. The encoded genes contained a hi  ...[more]

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