Genomics

Dataset Information

300

DNA methylation changes induced by overexpression of IDH1mut or treatment with 2HG in the sorted mouse bone marrow cells


ABSTRACT: Mutations in the enzymes IDH1 and IDH2 have been identified in a wide variety of tumors like glioma, chondrosarcoma, thyroid cancer, lymphoma, melanoma, and in acute myeloid leukemia. Mutated IDH1/2 produces the metabolite 2-hydroxyglutarate (2HG), which interferes with epigenetic regulation of gene expression, and thus may promote tumorigenesis. HoxA9 immortalised bone marrow cells from C57BJ/6 mice were tranduced with either empty vector or pSF91-IDH1wt-IRES-EGFP, or pSF91-IDH1mut-IRES-GFP and transplanted in irradiated recipient mice. Alternatively, HoxA9 immortalised cells transduced with empty vector transplanted mice were treated with R-2HG at dose of 1mg/day for four weeks. Four weeks after transplantation/treatment GFP+ cells were sorted from mouse bone marrow, from which total DNA was extracted and subjected to Reduced Representation Bisulfite Sequencing (RRBS) to determine the genome-wide methylation signature.

ORGANISM(S): Mus musculus  

SUBMITTER: Michael Heuser   Anuhar Chaturvedi 

PROVIDER: E-GEOD-77828 | ArrayExpress | 2016-06-01

SECONDARY ACCESSION(S): GSE77828SRP069897PRJNA311700

REPOSITORIES: GEO, ArrayExpress, ENA

Similar Datasets

2015-09-21 | E-GEOD-72247 | ArrayExpress
2012-07-22 | E-GEOD-38687 | ArrayExpress
2016-06-01 | E-GEOD-77594 | ArrayExpress
2013-05-08 | E-GEOD-46700 | ArrayExpress
2015-09-01 | E-GEOD-55159 | ArrayExpress
2014-10-01 | E-GEOD-59917 | ArrayExpress
2014-10-01 | E-GEOD-59916 | ArrayExpress
2012-11-20 | E-GEOD-41912 | ArrayExpress
2016-01-11 | E-GEOD-71039 | ArrayExpress
2015-04-21 | E-GEOD-68027 | ArrayExpress