Dataset Information


Expression data from CD1d tetramer-sorted b-GL122/LGL1–specific human T cells or iNKT cells

ABSTRACT: The ability to detect and isolate bGL1-22/LGL1–specific human type II NKT cells allowed us to compare the global gene expression profiles of these cells with type I NKT cells using microarray analysis. Principal component analysis revealed that the gene expression profile signature for bGL1-22 and LGL1-specific T cells both before and after activation with anti-CD3/CD28 beads is distinct from that of type I NKT cells. RNA from CD1d tetramer-sorted b-GL122/LGL1–specific T cells or iNKT cells was amplified, labeled, and hybridized on the Affymetrix Human Genome U133 Plus 2.0 microarray chips. The data were analyzed with GeneSpring GX 12.5 (Agilent Technologies)

ORGANISM(S): Homo sapiens  

SUBMITTER: Rakesh Verma  

PROVIDER: E-GEOD-77909 | ArrayExpress | 2016-02-16



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Type II NKT-TFH cells against Gaucher lipids regulate B-cell immunity and inflammation.

Nair Shiny S   Boddupalli Chandra Sekhar CS   Verma Rakesh R   Liu Jun J   Yang Ruhua R   Pastores Gregory M GM   Mistry Pramod K PK   Dhodapkar Madhav V MV  

Blood 20141211 8

Chronic inflammation including B-cell activation is commonly observed in both inherited (Gaucher disease [GD]) and acquired disorders of lipid metabolism. However, the cellular mechanisms underlying B-cell activation in these settings remain to be elucidated. Here, we report that β-glucosylceramide 22:0 (βGL1-22) and glucosylsphingosine (LGL1), 2 major sphingolipids accumulated in GD, can be recognized by a distinct subset of CD1d-restricted human and murine type II natural killer T (NKT) cells.  ...[more]

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