Transcriptomics

Dataset Information

33

LET-dependent change in rice gene expression profile after heavy-ion beam irradiation [LET]


ABSTRACT: Linear energy transfer (LET) is an important factor affecting several aspects of the irradiation effect, e.g. cell survival and mutation frequency, making the heavy-ion beam an effective mutagen. To study the mechanisms behind LET-dependent effects, expression profiling was performed after heavy-ion beam irradiation of imbibed rice seeds. Array-based experiments at three time points (0.5, 1, 2 h after the irradiation) revealed that the number of up- or down-regulated genes was highest 2 h after irradiation. Array-based experiments with four different LETs at 2 h after irradiation identified LET-independent regulated genes that were up/down-regulated regardless of the value of LET; LET-dependently regulated genes, whose expression level increased with the rise of LET value, were also identified. Oryza sativa L. 'Nipponbare' seeds were imbibed for 3 days. The seeds were irradiated with 22.5 or 50 keV/μm C-ion or 63 or 80 keV/μm Ne-ion at a dose of 15 Gy. Gene expressions of irradiated and unirradiated embryos were measured at 2 hours after irradiation. Three independent experiments were performed at each time and LET.

ORGANISM(S): Oryza sativa  

SUBMITTER: Kotaro Ishii   Tomoko Abe 

PROVIDER: E-GEOD-78994 | ArrayExpress | 2016-07-01

SECONDARY ACCESSION(S): GSE78994PRJNA314603

REPOSITORIES: GEO, ArrayExpress

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