Genomics

Dataset Information

236

Direct GR binding sites potentiate clusters of TF binding across the human genome [6]


ABSTRACT: The glucocorticoid receptor (GR) binds the human genome at >10,000 sites, but only regulates the expression of hundreds of genes. To determine the functional effect of each site, we measured the glucocorticoid (GC) responsive activity of nearly all GR binding sites (GBSs) captured using chromatin immunoprecipitation (ChIP) in A549 cells. 13% of GBSs assayed had GC-induced activity. The responsive sites were defined by direct GR binding via a GC response element (GRE) and exclusively increased reporter- gene expression. Meanwhile, most GBSs lacked GC-induced reporter activity. The non-responsive sites had epigenetic features of steady state enhancers and clustered around direct GBSs. Together, our data support a model in which clusters of GBSs observed with ChIP-seq reflect interactions between direct and tethered GBSs over tens of kilobases. We further show that those interactions can synergistically modulate the activity of direct GBSs, and may therefore play a major role in driving gene activation in response to GCs. Glucoroticoid receptor binding site chip-seq libraries were cloned into STARR-seq for massively parallel functioal analysis. The results were confirmed by ChIP-Exo performed on the GR in A549 cells treated with 100 nM dexamethasone for one hour. This dataset [6] contains the ChIP exo data from cells treated with 100 nM DEX.

ORGANISM(S): Homo sapiens  

SUBMITTER: Ian C McDowell   Timothy E Reddy  Lingyun Song  Christopher Vockley  Christopher M Vockley  Alexias Safi  William H Majoros  Gregory E Crawford   Anthony M D'Ippolito 

PROVIDER: E-GEOD-79429 | ArrayExpress | 2016-08-25

SECONDARY ACCESSION(S): GSE79429SRP072111PRJNA315839

REPOSITORIES: GEO, ArrayExpress, ENA

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