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Transcriptomics

Dataset Information

8

Expression data from infant ALL with MLL-AF4 rearrangement


ABSTRACT: MLL-AF4+ blasts from infant B-ALL, CB-derived CD34+CD38-CD19-CD33- HSC, CB-derived CD34+CD19+CD33- B-cell HPCs and CB-derived CD34+CD33+CD19- myeloid HPCs. We used microarray to estudy gene expression profile comparing ALL vs HSC, HPC and myeloid HPSC. We used highly FACS-purified (purity>98%) MLL-AF4+ blasts from infant B-ALL, CB-derived CD34+CD38-CD19-CD33- HSC, CB-derived CD34+CD19+CD33- B-cell HPCs and CB-derived CD34+CD33+CD19- myeloid HPCs. For each independent sample technical duplicates were always performed. Total RNA was extracted using TRIol reagent, and quantified on a Nanodrop spectrophotometer and Bioanalyzer. High-quality RNA was reverse transcribed and the obtained cDNA was used as a template to synthesize biotinylanted cDNA, then was fragmented and hybridized as duplicates/triplicates to HG-U133 plus2.0 GeneChips (Affymetrix) according to manufacturer's guideline.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michela Bardini   Gianni Cazzaniga  antonio agraz 

PROVIDER: E-GEOD-79450 | ArrayExpress | 2016-03-22

SECONDARY ACCESSION(S): GSE79450PRJNA315856

REPOSITORIES: GEO, ArrayExpress

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