Genomics

Dataset Information

299

Genome-wide DNA methylation profiling of mouse liver


ABSTRACT: To determine whether differences between background strains or housing conditions altered the hepatic methylome, We report the generation and analysis of genome-wide DNA methylation profiles at nucleotide resolution in mouse liver from two male mice on a mixed background (mixed-1, mixed-2) and two males on a pure Black-6 (B6-1, B62) background. Using Enhanced high-throughput Reduced Representation Bisulfite Sequencing (ERRBS), we enriched CpG islands in mouse liver, and covered a representative sampling of conserved non-coding elements, transposons and other genomic features, for mouse liver. We found that the total CpG methylation of each methylome was strikingly similar among the 4 mouse liver samples from two different genetic backgrounds. Analysis of all CpG sites with at least 10x coverage showed a bimodal distribution of methylation, with all samples having 25% of hypermethylated CpG sites and 60% as hypomethylated CpG sites. Given the high percent of genome coverage and robust depth at single nucleotide level, these datasets provide a resource for investigation into changes in DNA methylation patterns in liver disease, tumorigenesis and regeneration. Ehanced reduced representation bisulfite sequencing (MspI 70~320bp size fraction) of liver tissue

ORGANISM(S): Mus musculus  

SUBMITTER: Chi Zhang   Kirsten Sadler Edepli  Amaia Lujambio  Kirsten S Edepli 

PROVIDER: E-GEOD-80761 | ArrayExpress | 2016-06-13

SECONDARY ACCESSION(S): GSE80761SRP074115PRJNA319939

REPOSITORIES: GEO, ArrayExpress, ENA

Similar Datasets

2014-08-22 | E-GEOD-55569 | ArrayExpress
2012-06-01 | E-GEOD-37454 | ArrayExpress
2015-03-31 | E-GEOD-61161 | ArrayExpress
2014-01-27 | E-GEOD-45343 | ArrayExpress
2011-03-16 | E-GEOD-27003 | ArrayExpress
2015-01-01 | S-EPMC4650678 | BioStudies
2012-07-22 | E-GEOD-38687 | ArrayExpress
2014-01-27 | E-GEOD-51031 | ArrayExpress
1000-01-01 | S-EPMC5401809 | BioStudies
1000-01-01 | S-EPMC3749180 | BioStudies