Transcriptomics

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4

Role of σ54 in the regulation of genes involved in biogenesis of type I and type IV pili in Xylella fastidiosa


ABSTRACT: The phytopathogen Xylella fastidiosa produces two classes of pili, long type IV pili and short type I pili, which are involved in motility and adhesion. In this work, we have investigated the role of σ54 factor and its involvement in the regulation of fimbrial biogenesis in X. fastidiosa. An rpoN null mutant was constructed from citrus strain J1a12, and analyses of global gene expression profile by microarrays comparing the wild type and rpoN mutant strains showed that few genes exhibited differential expression. At least one gene, pilA1 (XF2542), which encodes the structural pilin protein of type IV fimbriae, showed decreased expression in the rpoN mutant whereas an operon encoding proteins of type I fimbriae were twofold more expressed in the mutant. Quantitative real time RT-PCR (qRT-PCR) analysis confirmed that pilA1 transcript was significantly reduced in the rpoN mutant. The transcriptional start site of pilA1 was determined by primer extension, and a canonical σ54-dependent promoter was found upstream of the start site. Genome sequence analysis of X. fastidiosa revealed five paralogues of pilA, but microarray and qRT-PCR data demonstrated that only the pilA1 transcript was significantly affected in the rpoN mutant. The rpoN mutant made more biofilm than the wild type strain and presented a cell-cell aggregative phenotype. These results indicate that σ54 regulates biofilm formation, probably via differential regulation of genes involved in type IV and type I fimbrial biogenesis. Direct comparison between wild type strain J1a12 and mutant strain rpoN-.

ORGANISM(S): Xylella fastidiosa  

SUBMITTER: Tie Koide  Jose F da Silva Neto  Marilis V Marques  Suely L Gomes 

PROVIDER: E-GEOD-8097 | ArrayExpress | 2010-06-25

SECONDARY ACCESSION(S): GSE8097PRJNA100951

REPOSITORIES: GEO, ArrayExpress

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Role of sigma54 in the regulation of genes involved in type I and type IV pili biogenesis in Xylella fastidiosa.

da Silva Neto José F JF   Koide Tie T   Abe Cecília M CM   Gomes Suely L SL   Marques Marilis V MV  

Archives of microbiology 20071106 3


The phytopathogen Xylella fastidiosa produces long type IV pili and short type I pili involved in motility and adhesion. In this work, we have investigated the role of sigma factor sigma(54) (RpoN) in the regulation of fimbrial biogenesis in X. fastidiosa. An rpoN null mutant was constructed from the non-pathogenic citrus strain J1a12, and microarray analyses of global gene expression comparing the wild type and rpoN mutant strains showed few genes exhibiting differential expression. In particul  ...[more]

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