Transcriptomics

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Transcriptomes of mouse PGCLCs isolated from 6-day culture embryoid bodies were compared with transcriptomes of their precur cells (ESCs, iPSCs, and EpiLCs) and E12.5 in vivo mouse PGCs


ABSTRACT: Transcriptomes of mouse E12.5 primordial germ cells (PGCs), primordial germ cell-like cells (PGCLCs) isolated from 6-day culture embryoid bodies, and the precursor pluripotent stem cells [embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)] and epiblast-like cells (EpiLCs) Total RNA was isolated from FACS-enriched, SSEA1+/CD61+ double-positive PGCs and PGCLCs. RNA was also isolated from ESC, iPSC, and EpiLC cultured without enrichment. Transcriptomes were determined using Affymetrix microarray.

ORGANISM(S): Mus musculus  

SUBMITTER: Toshi Shioda   Haley Ellis  Keiko Shioda  Jente M Stel  Norikatsu Miyoshi 

PROVIDER: E-GEOD-80983 | ArrayExpress | 2016-08-22

SECONDARY ACCESSION(S): GSE80983PRJNA320224

REPOSITORIES: GEO, ArrayExpress

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Publications

Erasure of DNA methylation, genomic imprints, and epimutations in a primordial germ-cell model derived from mouse pluripotent stem cells.

Miyoshi Norikatsu N   Stel Jente M JM   Shioda Keiko K   Qu Na N   Odajima Junko J   Mitsunaga Shino S   Zhang Xiangfan X   Nagano Makoto M   Hochedlinger Konrad K   Isselbacher Kurt J KJ   Shioda Toshi T  

Proceedings of the National Academy of Sciences of the United States of America 20160802 34


The genome-wide depletion of 5-methylcytosines (5meCs) caused by passive dilution through DNA synthesis without daughter strand methylation and active enzymatic processes resulting in replacement of 5meCs with unmethylated cytosines is a hallmark of primordial germ cells (PGCs). Although recent studies have shown that in vitro differentiation of pluripotent stem cells (PSCs) to PGC-like cells (PGCLCs) mimics the in vivo differentiation of epiblast cells to PGCs, how DNA methylation status of PGC  ...[more]

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