Transcriptomics

Dataset Information

463

A long noncoding RNA regulates sister chromatid cohesion [RNA-seq]


ABSTRACT: Long noncoding RNAs (lncRNAs) have appeared to be involved in the most diverse cellular processes through multiple mechanisms. Here we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), transcriptionally activated by MYC, which is upregulated in multiple cancer types. The expression of CONCR is cell cycle-regulated, and it is required for cell cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication. These findings suggest a novel mechanism of action for CONCR in the modulation of DDX11 enzymatic activity, unveiling the direct involvement of a lncRNA in the establishment of sister chromatid cohesion. Characterization of the function of the long noncoding RNA CONCR. HCT116 p53-/- cells were left untreated (0h) or treated with the DNA damaging drug 5-FU for 4h and 12h.

ORGANISM(S): Homo sapiens  

SUBMITTER: Alejandro Athie   Dannys J Martínez-Herrera  Alicia Amadoz  Sanjay K Bharti  Robert M Brosh  Jovanna González  Maite Huarte  Francesco P Marchese  Ivan Raimondi  Elena Grossi  Oskar Marín-Béjar 

PROVIDER: E-GEOD-81601 | ArrayExpress | 2016-08-03

SECONDARY ACCESSION(S): GSE81601SRP075373PRJNA322065

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications


Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggest  ...[more]

Similar Datasets

2016-08-03 | E-GEOD-81599 | ArrayExpress
| PRJNA106243 | ENA
| GSE80007 | GEO
2008-07-24 | GSE11527 | GEO
| GSE83959 | GEO
2014-08-05 | E-GEOD-59376 | ArrayExpress
2010-06-10 | E-GEOD-8270 | ArrayExpress
2007-07-13 | GSE8270 | GEO
2012-11-22 | E-GEOD-42458 | ArrayExpress
| PRJNA322064 | ENA