Transcriptomics

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Transcription profiling of human U937 lymphoma cells treated with paeony root (Paeoniae radix) (time series)


ABSTRACT: Paeoniflorin (PF) isolated from paeony root (Paeoniae radix) has been used as an herbal medicine in East Asis for its anti-allergic, anti-inflammatory, and immunoregulatory effects. PF is known to be a chemical heat shock protein (HSP) inducer. The effects on the gene expression in human lymphoma U937 cells treated with PF were investigated using by an Affymetrix GeneChip system. PF treatment induced Hsp70 expression in U937 cells in a dose- and time-dependent manner as shown in Western blot analysis. When the cells were treated with PF (160 μg/ml; 30 min), 41 up-regulated and 23 down-regulated genes were identified. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with paeoniflorin (0.16 mg/ml; 30 min) and followed by incubation for 0, 3, and 6 h at 37°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Yoshiaki Tabuchi  

PROVIDER: E-GEOD-8228 | ArrayExpress | 2008-06-16

SECONDARY ACCESSION(S): GSE8228PRJNA101155

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells.

Salunga Thucydides L TL   Tabuchi Yoshiaki Y   Takasaki Ichiro I   Feril Loreto B LB   Zhao Qing-Li QL   Ohtsuka Kenzo K   Tsuneyama Koichi K   Kondo Takashi T  

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group 20070901 6


Paeoniflorin (PF) isolated from peony root (Paeoniae radix) has been used as a herbal medicine in East Asia for its anti-allergic, anti-inflammatory, and immunoregulatory effects. PF is known to cause apoptosis and to be a chemical heat shock protein (HSP) inducer. With this information, the effects on the gene expression in human leukemia U937 cells treated with PF were investigated.U937 cells, a human myelomonocytic cell line, were treated with PF at different concentrations (0-640 microg/ml).  ...[more]

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