Genomics

Dataset Information

297

Functional analysis of AEBP2, a PRC2 Polycomb protein, reveals a Trithorax phenotype in embryonic development and in ES cells


ABSTRACT: The Polycomb repressive complexes PRC1 and PRC2 are key mediators of heritable gene silencing in multicellular organisms. Here we characterize AEBP2, a known PRC2 cofactor which, in vitro, has been shown to stimulate PRC2 activity. We show that AEBP2 localises specifically to PRC2 target loci, including the inactive X chromosome. Proteomic analysis confirms that AEBP2 associates exclusively with PRC2 complexes. However, analysis of embryos homozygous for a targeted mutation of Aebp2 unexpectedly revealed a Trithorax phenotype, normally linked to antagonism of Polycomb function. Consistent with this we observe elevated levels of PRC2 mediated histone H3K27 methylation at target loci in Aebp2 mutant embryonic stem cells. We further demonstrate that mutant ES cells assemble atypical hybrid PRC2 sub-complexes, potentially accounting for enhancement of Polycomb activity, and suggesting that AEBP2 normally plays a role in defining the mutually exclusive composition of PRC2 sub-complexes. H3K27me3, SUZ12, and AEBP2 ChIP-Seq in wild-type and AEBP2 KO mouse ESCs, biological replicates, pre-cleared chromatin as input, additionally FS2 ChIP-Seq in cells with FS2 tagged AEBP2, HiSeq2000

ORGANISM(S): Mus musculus  

SUBMITTER: Joanna F McGouran   Haruhiko Koseki  Jonathan Godwin  Michal R Gdula  Shoumo Bhattacharya  Benedikt M Kessler  Anne Grijzenhout  Sarah Cooper  Neil Brockdorff  Michal R. Gdula  Dorota Szumska 

PROVIDER: E-GEOD-83082 | ArrayExpress | 2016-08-02

SECONDARY ACCESSION(S): SRP076239GSE83082PRJNA324709

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Functional analysis of AEBP2, a PRC2 Polycomb protein, reveals a Trithorax phenotype in embryonic development and in ESCs.

Grijzenhout Anne A   Godwin Jonathan J   Koseki Haruhiko H   Gdula Michal Ryszard MR   Szumska Dorota D   McGouran Joanna F JF   Bhattacharya Shoumo S   Kessler Benedikt M BM   Brockdorff Neil N   Cooper Sarah S  

Development (Cambridge, England) 20160617 15


The Polycomb repressive complexes PRC1 and PRC2 are key mediators of heritable gene silencing in multicellular organisms. Here, we characterise AEBP2, a known PRC2 co-factor which, in vitro, has been shown to stimulate PRC2 activity. We show that AEBP2 localises specifically to PRC2 target loci, including the inactive X chromosome. Proteomic analysis confirms that AEBP2 associates exclusively with PRC2 complexes. However, analysis of embryos homozygous for a targeted mutation of Aebp2 unexpected  ...[more]

Similar Datasets

2015-01-22 | E-GEOD-55630 | ArrayExpress
2014-05-27 | E-GEOD-55697 | ArrayExpress
2016-04-01 | E-GEOD-76467 | ArrayExpress
2015-06-25 | E-GEOD-62666 | ArrayExpress
2014-07-03 | E-GEOD-58022 | ArrayExpress
2014-07-03 | E-GEOD-58019 | ArrayExpress
2009-11-30 | E-GEOD-19076 | ArrayExpress
2008-11-01 | E-GEOD-13084 | ArrayExpress
2016-05-17 | E-GEOD-74330 | ArrayExpress
2012-11-17 | E-GEOD-41609 | ArrayExpress