Transcriptomics

Dataset Information

346

Elucidation of Transcriptome-Wide MicroRNA Binding Sites in Human Cardiac Tissues by Ago2 HITS-CLIP


ABSTRACT: We deciphered Ago2:RNA interactions in post-mortem human heart tissues using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Profiling AGO2-associated RNAs in cardiac tissues obtained from 6 donors with heart diseases of differing etiologies

ORGANISM(S): Homo sapiens  

SUBMITTER: Ryan M Spengler   Ryan L Boureau  Ryan L Boudreau 

PROVIDER: E-GEOD-83410 | ArrayExpress | 2016-08-09

SECONDARY ACCESSION(S): SRP076625GSE83410PRJNA325822

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Elucidation of transcriptome-wide microRNA binding sites in human cardiac tissues by Ago2 HITS-CLIP.

Spengler Ryan M RM   Zhang Xiaoming X   Cheng Congsheng C   McLendon Jared M JM   Skeie Jessica M JM   Johnson Frances L FL   Davidson Beverly L BL   Boudreau Ryan L RL  

Nucleic acids research 20160714 15


MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, t  ...[more]

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