Transcriptomics

Dataset Information

4

Generation and purification of Th1 and Th2 effector and memory CD4+ T cells


ABSTRACT: Naïve CD4+ T cells were isolated from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice (Kaye et al., Nature 1989;341:746, Wright et al, Blood 2001;97:2278) that recognize a peptide of pigeon cytochrome C in the context of I-Ek and express CD44lo, CD62Lhi, CD45RBhi, and CD25-. After 4 days in vitro stimulation with antigen presenting cells (APC) under either Th1 or Th2 condition, naïve cells become Th1 or Th2 effector cells expressing CD44hi, CD62L lo, CD45RBhi, and CD25+. Additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-). These rested effector cells were adaptively transferred into thymectomized, lethally irradiated, and T cell depleted bone marrow reconstituted mice and memory cells were isolated after 4-12 weeks by flow sort. Generation and purification of Th1 and Th2 effector and memory CD4+ T cells of 42 samples.

ORGANISM(S): Mus musculus  

SUBMITTER: Kevin G Becker   W H Lee  Susan L Swain  N P Weng  Susanne Golech  K K McKinstry  Gail Huston 

PROVIDER: E-GEOD-8352 | ArrayExpress | 2010-06-10

SECONDARY ACCESSION(S): GSE8352PRJNA101367

REPOSITORIES: GEO, ArrayExpress

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Publications

Rapid default transition of CD4 T cell effectors to functional memory cells.

McKinstry K Kai KK   Golech Susanne S   Lee Won-Ha WH   Huston Gail G   Weng Nan-Ping NP   Swain Susan L SL  

The Journal of experimental medicine 20070827 9


The majority of highly activated CD4 T cell effectors die after antigen clearance, but a small number revert to a resting state, becoming memory cells with unique functional attributes. It is currently unclear when after antigen clearance effectors return to rest and acquire important memory properties. We follow well-defined cohorts of CD4 T cells through the effector-to-memory transition by analyzing phenotype, important functional properties, and gene expression profiles. We find that the tra  ...[more]

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