Transcriptomics,Multiomics

Dataset Information

9

Chip-chip from pro-B cells with H3K4me3, H3K4me2 and H3K9ac


ABSTRACT: The transcription factor Pax5 represses B-lineage-inappropriate genes and activates B-cell-specific genes in B-lymphocytes. Here we have identified 170 novel Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expression in pro-B and mature B cells. Expression of half of the Pax5-activated genes is either absent or significantly reduced upon Pax5 loss in plasma cells. Direct Pax5 target genes were identified based on their protein synthesis-independent activation by a Pax5-estrogen receptor fusion protein. Chromatin immunoprecipitation (ChIP) of Pax5 together with chromatin profiling by ChIP-on-chip analysis demonstrated that Pax5 directly activates the chromatin at promoters or putative enhancers of Pax5 target genes. The novel Pax5-activated genes code for key regulatory and structural proteins involved in B cell signaling, adhesion, migration, antigen presentation and germinal center B cell formation, thus revealing a complex regulatory network, which is activated by Pax5 to control B cell development and function. Keywords: Chip-chip, cell type comparison comparison of Pax5-/-Rag2-/- vs Rag2-/- pro-B cells

OTHER RELATED OMICS DATASETS IN: PRJNA101555PRJNA105349PRJNA101541PRJNA105351

ORGANISM(S): Mus musculus  

SUBMITTER: Shane McManus   Meinrad Busslinger 

PROVIDER: E-GEOD-8472 | ArrayExpress | 2007-08-23

SECONDARY ACCESSION(S): GSE8472PRJNA101555

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-8472.README.txt Txt
E-GEOD-8472.idf.txt Idf
E-GEOD-8472.processed.1.zip Processed
E-GEOD-8472.sdrf.txt Txt
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Publications

Transcription factor Pax5 activates the chromatin of key genes involved in B cell signaling, adhesion, migration, and immune function.

Schebesta Alexandra A   McManus Shane S   Salvagiotto Giorgia G   Delogu Alessio A   Busslinger Georg A GA   Busslinger Meinrad M  

Immunity 20070719 1


The transcription factor Pax5 represses B lineage-inappropriate genes and activates B cell-specific genes in B lymphocytes. Here we have identified 170 Pax5-activated genes. Conditional mutagenesis demonstrated that the Pax5-regulated genes require continuous Pax5 activity for normal expression in pro-B and mature B cells. Expression of half of the Pax5-activated genes is either absent or substantially reduced upon Pax5 loss in plasma cells. Direct Pax5 target genes were identified based on thei  ...[more]

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