Transcriptomics

Dataset Information

10

Transcription profiling of human eosinophilic esophagitis biopsies, cultured and stimulated with IL-13


ABSTRACT: 3 eosinophilic esophagitis biopsies, cultured and stimulated with IL-13 : each of them was either left unstimulated or stimulated (100ng for 48h); We used microarray to uncover the IL-13-induced genes in esophageal epithelial cells of the esophagus Experiment Overall Design: 3 biopsies from EE patients were obtained and primary epithelial cell were cultured and either left unstimulated or stimulated with IL-13 followed by RNA extraction and hybridization on Affymetrix microarrays.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Marc E. Rothenberg  

PROVIDER: E-GEOD-8853 | ArrayExpress | 2008-06-20

SECONDARY ACCESSION(S): GSE8853PRJNA102205

REPOSITORIES: GEO, ArrayExpress

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Publications

IL-13 involvement in eosinophilic esophagitis: transcriptome analysis and reversibility with glucocorticoids.

Blanchard Carine C   Mingler Melissa K MK   Vicario Maria M   Abonia J Pablo JP   Wu Yi Ying YY   Lu Thomas X TX   Collins Margaret H MH   Putnam Philip E PE   Wells Susanne I SI   Rothenberg Marc E ME  

The Journal of allergy and clinical immunology 20071201 6


BACKGROUND: Eosinophilic esophagitis (EE) is an emerging worldwide disease that mimics gastroesophageal reflux disease. Early studies have established that esophageal eosinophilia occurs in association with T(H)2 allergic responses, and we recently identified an EE-specific esophageal transcriptome that included eotaxin-3. OBJECTIVE: We sought to determine the mechanism by which this T(H)2 response leads to EE. METHODS: Real-time PCR and microarray analysis were performed on RNA extracted from e  ...[more]

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