Transcriptomics

Dataset Information

3

Transcription profiling by array of Arabidopsis after treatment with imidazolinone


ABSTRACT: Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to imidazolinone (Arsenal) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered. Experiment Overall Design: Surfactant (preference 0.25%) treated plants were used as carrier control group and EC50 concentration of imidazolinone was used as the herbicide treatment group. Each of the control and treatment group consisted of 3 biological replicates and each biological replicates comprised leaves from 10 individual plants. RNA was extracted at 24h post treatment to study the transcriptional alterations caused by the herbicide treatment.

REANALYSED by: E-GEOD-8925

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Malay Das  

PROVIDER: E-GEOD-8925 | ArrayExpress | 2009-08-07

SECONDARY ACCESSION(S): GSE8925PRJNA102335

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-8925.idf.txt Idf
E-GEOD-8925.processed.1.zip Processed
E-GEOD-8925.raw.1.zip Raw
E-GEOD-8925.sdrf.txt Txt
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Publications

A composite transcriptional signature differentiates responses towards closely related herbicides in Arabidopsis thaliana and Brassica napus.

Das Malay M   Reichman Jay R JR   Haberer Georg G   Welzl Gerhard G   Aceituno Felipe F FF   Mader Michael T MT   Watrud Lidia S LS   Pfleeger Thomas G TG   Gutiérrez Rodrigo A RA   Schäffner Anton R AR   Olszyk David M DM  

Plant molecular biology 20091231 4-5


In this study, genome-wide expression profiling based on Affymetrix ATH1 arrays was used to identify discriminating responses of Arabidopsis thaliana to five herbicides, which contain active ingredients targeting two different branches of amino acid biosynthesis. One herbicide contained glyphosate, which targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), while the other four herbicides contain different acetolactate synthase (ALS) inhibiting compounds. In contrast to the herbicide cont  ...[more]

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