Transcriptomics

Dataset Information

4

Gene expression profiles of primary tumors with radiotherapy and lung metastases in mouse squamous cell carcinoma model.


ABSTRACT: To examine whether the local carbon ion radiotherapy affects the characteristics of the metastatic tumors, the expression profiles of the primary tumors and the lung metastases were studied in a mouse squamous cell carcinoma model by applying local radiotherapy with no irradiation (negative control), gamma-ray irradiation (reference beam), and carbon-ion irradiation. Keywords: mouse, squamous cell carcinoma, primary tumor, lung metastases, radiotherapy, carbon ion, gamma ray A highly metastatic mouse squamous cell carcinoma NR-S1 was implanted into the hind leg of synergetic C3H/HeNrs mice and irradiated with 5 Gy of carbon ion beam. 8 Gy of gamma ray was used as a reference beam. At 2 weeks after the irradiation, the lung tissue was sampled. In order to collect samples of primary tumors, the tumors were implanted in other mice and irradiated in the same manner, and the primary tumors were collected at 1 week after the irradiation. The tumor cells of the primary and metastatic tumors were collected by laser microdissection, and oligonucleotide microarray analysis of the irradiated primary tumors and the metastatic tumors were all performed in comparison to the non-irradiated primary tumor by two-color methods.

ORGANISM(S): Mus musculus  

SUBMITTER: Kaori Imadome   Tomoaki Tamaki  Mayumi Iwakawa 

PROVIDER: E-GEOD-9305 | ArrayExpress | 2009-04-22

SECONDARY ACCESSION(S): GSE9305PRJNA102955

REPOSITORIES: GEO, ArrayExpress

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Publications

Application of carbon-ion beams or gamma-rays on primary tumors does not change the expression profiles of metastatic tumors in an in vivo murine model.

Tamaki Tomoaki T   Iwakawa Mayumi M   Ohno Tatsuya T   Imadome Kaori K   Nakawatari Miyako M   Sakai Minako M   Tsujii Hirohiko H   Nakano Takashi T   Imai Takashi T  

International journal of radiation oncology, biology, physics 20090501 1


PURPOSE: To clarify how carbon-ion radiotherapy (C-ion) on primary tumors affects the characteristics of subsequently arising metastatic tumor cells. METHODS AND MATERIALS: Mouse squamous cell carcinomas, NR-S1, in synergic C3H/HeMsNrs mice were irradiated with a single dose of 5-50 Gy of C-ion (290 MeV per nucleon, 6-cm spread-out Bragg peak) or gamma-rays ((137)Cs source) as a reference beam. The volume of the primary tumors and the number of metastatic nodules in lung were studied, and histol  ...[more]

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