Transcriptomics

Dataset Information

3

Response of L. lactis faced to S. cerevisiae


ABSTRACT: The response of L. lactis to the presence of S. cerevisiae was analyzed during the exponential growth phase in fermentors in defined growth conditions. Although no growth kinetic difference was observed between the pure and mixed culture of L. lactis, the mRNA level of genes was significantly modified. More particularly, a strong reorientation of pyrimidine metabolism was observed when L. lactis was grown in the mixed culture. Keywords: microbial interaction, time course A pure culture of L. lactis was conducted in parallel with a co-culture of L. lactis and S. cerevisiae. For the transcriptomic analysis, the mixed culture (test condition, 2.5 hours of culture, B’) was compared to the pure culture (reference, 2.5 hours of culture, B) on the same slide at the same time. The mRNA level changes between 2 and 3 hours (A and C samples) flanking B sample (2.5 hours) in the pure culture were also determined on another slide. Total RNA was extracted from these samples and labelled cDNA (Cy3/Cy5) were prepared and hybridized on glass slides exhibiting 2004 amplicons specific of Lactococcus lactis IL1403 genes. 3 independent repetitions were performed.

ORGANISM(S): Lactococcus lactis  

SUBMITTER: Pascal Loubière   Muriel Cocaign-Bousquet  Mathieu Maligoy 

PROVIDER: E-GEOD-9436 | ArrayExpress | 2010-05-18

SECONDARY ACCESSION(S): GSE9436PRJNA103189

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome analysis of Lactococcus lactis in coculture with Saccharomyces cerevisiae.

Maligoy Mathieu M   Mercade Myriam M   Cocaign-Bousquet Muriel M   Loubiere Pascal P  

Applied and environmental microbiology 20071109 2


The study of microbial interactions in mixed cultures remains an important conceptual and methodological challenge for which transcriptome analysis could prove to be the essential method for improving our understanding. However, the use of whole-genome DNA chips is often restricted to the pure culture of the species for which the chips were designed. In this study, massive cross-hybridization was observed between the foreign cDNA and the specific Lactococcus lactis DNA chip. A very simple method  ...[more]

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