Transcriptomics

Dataset Information

3

Zea mays B73 stress treatments on UA 70mer oligo slides (Maize oligo array version 1.3 array A and B)


ABSTRACT: Maize seedling transcriptome responses to six abiotic perturbations (heat, cold, darkness, desiccation, salt, UV-B) and 2 controls were analyzed Keywords: comparitive transcriptome profiling Tissues were grown at Stanford University during the summer of 2005. RNA was extracted and purified, cDNA generated, and amplified cRNA was labeled with Cy3 or Cy5 dyes from 4 biological replicates of each treatment. Each replicate was composed of a pool of 10 similarly treated whole seedlings (with the endosperm and kernel removed). Two types of biological controls were employed: 1) seedlings germinated under constant 26ºC temperature, with 16 h light and adequate water and 2) field grown juvenile leaf samples Field seedlings emerged on day 6 or 7.

ORGANISM(S): Zea mays  

SUBMITTER: Virginia Walbot   John Fernandes  John F Fernandes  Darren Morrow  Paula Casati 

PROVIDER: E-GEOD-9453 | ArrayExpress | 2008-05-31

SECONDARY ACCESSION(S): GSE9453PRJNA103215

REPOSITORIES: GEO, ArrayExpress

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Publications

Distinctive transcriptome responses to adverse environmental conditions in Zea mays L.

Fernandes John J   Morrow Darren J DJ   Casati Paula P   Walbot Virginia V  

Plant biotechnology journal 20080708 8


SUMMARY: Maize seedling transcriptome responses to six abiotic perturbations (heat, cold, darkness, desiccation, salt, ultraviolet-B) were analysed. Approximately 7800 transcripts were expressed in one or more treatments compared with light-grown seedlings plus juvenile leaves from field-grown plants. Approximately 5200 transcripts were expressed in one or more treatments and absent in light-grown seedlings. Approximately 2000 transcripts were unique to one treatment. Salt and heat elicited the  ...[more]

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