Transcriptomics

Dataset Information

15

Transcription profiling by array of human mammary epithelial cells exposed to different environmental stresses: hypoxia, lactic acidosis, lactosis, acidosis and a combination of hypoxia and lactic acidosis


ABSTRACT: Human Mammalian Epithelial Cells (HMEC) were exposed to different environmental stresses, including hypoxia, lactic acidosis, the combination of hypoxia and lactic acidosis, lactosis , as well as acidosis. We used microarrays to examine the genomic programs of cells incubated under different microenvironments. Experiment Overall Design: HMEC cells were exposed to different environmental stresses and RNAs were extracted and put on Affymetrix microarrays. We gathered RNAs from cells grown in regular media (control), lactic acidosis, hypoxia, the combinatino of lactic acidosis and hypoxia, lactosis, as well as acidosis.

REANALYSED by: E-GEOD-9649

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Julia Ling-Yu Chen  

PROVIDER: E-GEOD-9649 | ArrayExpress | 2009-01-13

SECONDARY ACCESSION(S): GSE9649PRJNA103515

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

The genomic analysis of lactic acidosis and acidosis response in human cancers.

Chen Julia Ling-Yu JL   Lucas Joseph E JE   Schroeder Thies T   Mori Seiichi S   Wu Jianli J   Nevins Joseph J   Dewhirst Mark M   West Mike M   Chi Jen-Tsan JT  

PLoS genetics 20081205 12


The tumor microenvironment has a significant impact on tumor development. Two important determinants in this environment are hypoxia and lactic acidosis. Although lactic acidosis has long been recognized as an important factor in cancer, relatively little is known about how cells respond to lactic acidosis and how that response relates to cancer phenotypes. We develop genome-scale gene expression studies to dissect transcriptional responses of primary human mammary epithelial cells to lactic aci  ...[more]

Similar Datasets

2014-08-16 | E-GEOD-9649 | ExpressionAtlas
2012-02-27 | E-GEOD-29406 | ArrayExpress
2010-09-27 | E-GEOD-19123 | ArrayExpress
2008-12-18 | GSE9649 | GEO
2015-08-12 | E-GEOD-29406 | ExpressionAtlas
2012-02-27 | GSE29406 | GEO
2010-09-27 | GSE19123 | GEO
2015-12-10 | E-GEOD-61286 | ArrayExpress
| PRJNA103515 | ENA
2012-12-31 | E-GEOD-25525 | ArrayExpress