Dataset Information


RNAi expression experiments on Illumina BeadChip

ABSTRACT: Mouse embryonic stem cells (mES) were depleted of Klf2, Klf4, and Klf5 by ectopic expression of shRNA. Control RNAi was performed using shRNA against luciferase. At 2 days, 4 days and 6 days post-transfection of shRNA-encoding vectors, cells are harvested for RNA isolation. Keywords: RNAi expression, Mouse ES cells Knockdown: Klf2, Klf4, Klf5, Luciferase (Control);Time: 2 days post-transfection, 4 days post-transfection, 6 days post-transfection;Three independent experimental replicates for each experimental condition were performed.

ORGANISM(S): Mus musculus  

SUBMITTER: Huck H Ng  jun cai 

PROVIDER: E-GEOD-9775 | ArrayExpress | 2010-06-25



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A core Klf circuitry regulates self-renewal of embryonic stem cells.

Jiang Jianming J   Chan Yun-Shen YS   Loh Yuin-Han YH   Cai Jun J   Tong Guo-Qing GQ   Lim Ching-Aeng CA   Robson Paul P   Zhong Sheng S   Ng Huck-Hui HH  

Nature cell biology 20080210 3

Embryonic stem (ES) cells are unique in their ability to self-renew indefinitely and maintain pluripotency. These properties require transcription factors that specify the gene expression programme of ES cells. It has been possible to reverse the highly differentiated state of somatic cells back to a pluripotent state with a combination of four transcription factors: Klf4 is one of the reprogramming factors required, in conjunction with Oct4, Sox2 and c-Myc. Maintenance of self-renewal and pluri  ...[more]

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