Transcriptomics

Dataset Information

4

Msn2/4 mutant 3 hour exposure to zymolyase


ABSTRACT: We did transcription profiling on the effect of msn2 msn4 deletion, genes involved in several stress process Keywords: cell wall stress response S. cerevisiae (msn2/4 mutant) was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 °C and processed for RNA extraction. The total RNA from one culture was analyzed, and, in addition, for this sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to two DNA microarrays analyzed.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Javier Arroyo   Cesar Nombela  Clara Bermejo  Raul Garcia  Jose M Rodriguez-Peña 

PROVIDER: E-GEOD-9934 | ArrayExpress | 2010-06-19

SECONDARY ACCESSION(S): GSE9934PRJNA114675

REPOSITORIES: GEO, ArrayExpress

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Publications

The high osmotic response and cell wall integrity pathways cooperate to regulate transcriptional responses to zymolyase-induced cell wall stress in Saccharomyces cerevisiae.

García Raúl R   Rodríguez-Peña Jose M JM   Bermejo Clara C   Nombela César C   Arroyo Javier J  

The Journal of biological chemistry 20090220 16


The adaptation of Saccharomyces cerevisiae to situations in which cell wall integrity is seriously compromised mainly involves the cell wall integrity (CWI) pathway. However, in a recent work ( Bermejo, C., Rodriguez, E., García, R., Rodríguez-Peña, J. M., Rodríguez de la Concepción, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., and Arroyo, J. (2008) Mol. Biol. Cell 19, 1113-1124 ) we have demonstrated the co-participation of the high osmotic response (HOG) pathway to ensure yeast surviva  ...[more]

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