Transcriptomics

Dataset Information

2

Transcription profiling of human B-cells (BL41) for tc-, nt- and p-RNA half-life determination


ABSTRACT: RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis; We used microarrays to determine half-lives of mRNAs expressed in BL41 cells Experiment Overall Design: RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Lars Dölken  

PROVIDER: E-GEOD-9973 | ArrayExpress | 2009-10-20

SECONDARY ACCESSION(S): GSE9973PRJNA109051

REPOSITORIES: GEO, ArrayExpress

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