Transcription profiling of 5 tumor colorectal cancer biopsies vs normal on multiple platforms
ABSTRACT: Cross-platform target gene screening in colorectal cancer (CRC): we have compared 25 tumoural CRC biopsies against their normal counterpart in 30 hybridizations with a home-made cDNA array (CNIO oncochip) and 16 hybridisations with a custom oligoarray (Agilent Technologies).
Project description:Microarray technology was used to monitor the level of expression of 9,300 human genes at different time-points after infection of the type II alveolar A549 human cell line with HRSVs.
Project description:CD14+ purified bovine monoctye stimulation with CpG ODN 2007 vs. GpC ODN 2007, CpG 2007 vs. Control, GpC 2007 vs. Control and Media vs Control (Control is unstimluated CD14+ purified bovine monocytes at time zero). Before stimulation, the CD14+ purified bovine monocytes were rested for 20 h. Then the cells were stimulated for 4hrs.
Project description:Mice were acclimatized for 2 weeks and randomly assigned to treatment and control groups (five per group). Animals were given a single dose of 250, 50, 5 and 0.5 mg/kg isoproterenol by oral gavage alongside 0.9% saline (vehicle control group)and sacrificed after eight hours. Total mouse liver RNA was isolated and used for the microarry analysis on custom oligonucleotide DNA microarray, the HC ToxArray V1.2.<br><br>
Project description:A comparison of Ky mouse mutant soleus muscles versus wildtype soleus muscles. Hybridisation of 3 Mouse SGC 7.5k oligo slides, using 3 independent pools of 10 mice (5 male, 5 female)for both WT and mutant animals.
Project description:A comparison of Ostesy mutant soleus muscles to wildtype soleus muscles. Hybridisation of 3 Mouse SGC 7.5k oligo arrays, using 3 pools of 10 mice(5 male and 5 female) for mutant and WT.
Project description:Pregnant dams were treated with 0.1% or 0.04% 6-propyl-2-thiouracil (PTU) in drinking water continuously from day 13 post conception until weaning to produce hypothyroid pups. livers were collected from vehicle and 0.1% PTU treated pups at post-natal day (PND) 15 and mRNA from these was subjected to microarray analysis using Agilent high-density oligonucleotide chips.