Transcription profiling of leaves from wild type, fou2 mutant and tpc1-2 mutant Arabidopsis plants
ABSTRACT: Compare gene expression in the resting leaves of Arabidopsis thaliana WT with the mutants fou2 and tpc1-2. To analyse the contribution of the fou2 and tpc1-2 mutations in Arabidopsis thaliana to gene expression, transcript levels in the resting leaves of Arabidopsis were measured using 4 weeks-old plants grown in control conditions (long day).
The fatty acid oxygenation up-regulated 2 (fou2) mutant in Arabidopsis thaliana creates a gain-of-function allele in a non-selective cation channel encoded by the Two Pore Channel 1 (TPC1) gene. This mutant genetically implicates cation fluxes in the control of the positive feedback loop whereby jasmonic acid (JA) stimulates its own synthesis. In this study we observed extensive transcriptome reprogramming in healthy fou2 leaves closely resembling that induced by treatment with methyl jasmonate, ...[more]