Transcriptomics

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Transcription profiling of Listeria monocytogenes EGD-e after heat-shock for 3, 10, 20, and 40 min


ABSTRACT: L. monocytogenes EGD-e (Glaser et al., 2001) was grown in Brain Hearth Infusion Broth (Difco) with shaking (200 rpm, New Brunswick C24KC) using 10 ml culture medium in 100 ml conical flasks. An exponentially growing culture was used to inoculate 100 ml of fresh pre-warmed BHI broth in a 500 ml flask. This culture was incubated at 48°C (GFL Type 1083, 60 % shaking speed) and samples for RNA extraction were taken after 3, 10, 20, and 40 min.

INSTRUMENT(S): Generation III Array Scanner [Amersham]

ORGANISM(S): Listeria monocytogenes  

SUBMITTER: Torsten Hain  

PROVIDER: E-MEXP-1118 | ArrayExpress | 2007-10-09

REPOSITORIES: ArrayExpress

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Publications

The heat-shock response of Listeria monocytogenes comprises genes involved in heat shock, cell division, cell wall synthesis, and the SOS response.

van der Veen Stijn S   Hain Torsten T   Wouters Jeroen A JA   Hossain Hamid H   de Vos Willem M WM   Abee Tjakko T   Chakraborty Trinad T   Wells-Bennik Marjon H J MH  

Microbiology (Reading, England) 20071001 Pt 10


The food-borne pathogen Listeria monocytogenes has the ability to survive extreme environmental conditions due to an extensive interacting network of stress responses. It is able to grow and survive at relatively high temperatures in comparison with other non-sporulating food-borne pathogens. To investigate the heat-shock response of L. monocytogenes, whole-genome expression profiles of cells that were grown at 37 degrees C and exposed to 48 degrees C were examined using DNA microarrays. Transcr  ...[more]

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