Transcriptomics

Dataset Information

12

Transcription profiling of Drosophila expressing four mutant forms of MYOC (R342K, Q368X, D380N and K423E) to model Myocilin-Associated Glaucoma


ABSTRACT: We have established Drosophila melanogaster as a model system for ocular hypertension by expressing wild-type human myocilin (MYOC) in the Drosophila eye. Here, we have created transgenic flies that express four clinically relevant mutant forms of MYOC (R342K, Q368X, D380N and K423E) in their eyes using the gmr-Gal4/UAS binary system. We compare and identify human glaucoma candidate genes based on the transcription profiles of flies that express wt-MYOC or mutant-MYOCs.

REANALYSED by: E-MEXP-1179

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Mary Anna Carbone  

PROVIDER: E-MEXP-1179 | ArrayExpress | 2008-07-13

REPOSITORIES: ArrayExpress

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Publications

Overexpression of myocilin in the Drosophila eye activates the unfolded protein response: implications for glaucoma.

Carbone Mary Anna MA   Ayroles Julien F JF   Yamamoto Akihiko A   Morozova Tatiana V TV   West Steven A SA   Magwire Michael M MM   Mackay Trudy F C TF   Anholt Robert R H RR  

PloS one 20090116 1


Glaucoma is the world's second leading cause of bilateral blindness with progressive loss of vision due to retinal ganglion cell death. Myocilin has been associated with congenital glaucoma and 2-4% of primary open angle glaucoma (POAG) cases, but the pathogenic mechanisms remain largely unknown. Among several hypotheses, activation of the unfolded protein response (UPR) has emerged as a possible disease mechanism.We used a transgenic Drosophila model to analyze whole-genome transcriptional prof  ...[more]

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