Transcriptomics

Dataset Information

15

Transcription profiling of human prostate cancer cells, normal epithelial prostatic cells and stroma cells from patients in placebo, selenium, vitamin E or selenium and vitamin E treatment groups


ABSTRACT: 85 radical prostatectomy specimens (where 16 samples are in Placebo group (PL), 15 are in Selenium group (SE), 25 are in Vitamin E group (VE) and 27 are in Vitamin E & Selenium group (VS.Treatment groups: l-selenomethionine, 400 ug + placebo (vitamin E); vitamin E, 400 IU + placebo (l-selenomethionine); l-selenomethionine, 400 ug + vitamin E, 400 IU; placebos) were subjected to laser capture microdissection to isolate prostate cancer cells, normal epithelial prostatic cells, or stroma cells. RNA extraction and amplification were performed. Transcription profiling was conducted using the HG-U133A Affymetrix arrays.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

DISEASE(S): Prostate Cancer Tumor,Normal

SUBMITTER: Dimitra Tsavachidou  

PROVIDER: E-MEXP-1327 | ArrayExpress | 2008-07-01

REPOSITORIES: ArrayExpress

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Publications


Secondary analyses of two randomized, controlled phase III trials demonstrated that selenium and vitamin E could reduce prostate cancer incidence. To characterize pharmacodynamic and gene expression effects associated with use of selenium and vitamin E, we undertook a randomized, placebo-controlled phase IIA study of prostate cancer patients before prostatectomy and created a preoperative model for prostatectomy tissue interrogation.Thirty-nine men with prostate cancer were randomly assigned to  ...[more]

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