Transcription profiling of Salmonella enterica Typhimurium during infection of human epithelial cells
ABSTRACT: Epithelial cells are the first cell type Salmonella Typhimurium will encounter when infecting a host. Using DNA array techology we identified the Salmonella enterica Typhimurium genes regulated inside human epithelial cells and their variation through time.
Salmonella is the causative agent of a spectrum of human and animal diseases ranging from gastroenteritis to typhoid fever. It is a food--and water--borne pathogen and infects via ingestion followed by invasion of intestinal epithelial cells and phagocytic cells. In this study we employed a mutational approach to define the nutrients and metabolic pathways required by Salmonella enterica serovar Typhimurium during infection of a human epithelial cell line (HeLa). We deleted the key glycolytic ge ...[more]
Project description:NADPH dependent phagocytic oxidase, by producing hydroxyradicals such as hydrogen peroxide, is essential for host defense against Salmonella infection. We used gene array analysis to identify Salmonella enterica serovar Typhimurium genes regulated by NADPH dependent phagocytic oxidase intracellularly in comparison to those expressed in vitro by hydrogen peroxide.
Project description:The representative cell line, HT29, culture in hypoxic and normoxic conditions were used to perform the miRNA microarray. The chip assay and data analysis were entrusted to KangChen Bio-tech, Inc. (Shanghai, China) and all the miRNAs with more than 2-fold variation in different samples were identified. Detailed methods were performed as previously described24 using the miRCURY Hy3 labeling kit and the microRNA array software (Exiqon, Denmark).
Project description:We have carried out microarray-based gene expression analysis of cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen, from freshly prepared mouse mammary glands. RNA isolated from cell populations was co-hybridised with control mouse RNA on to an in-house (Breakthrough Breast Cancer Centre) cDNA mouse microarray containing 13825 features (NIA 15K Mouse cDNA clone set). LIMMA analysis was used to analyse data and determine changes in RNA expression levels.
Project description:To characterize early epigenetic events in breast carcinogenesis, we analyzed DNA methylation state of different stages of HMECs from pre-stasis to cancer cell lines using human promoter microarray
Project description:DNA microarrays were used to provide a global transcriptional profile of S. typhimurium during the intracellular growth phase of infection pathogenesis following infection of macrophages.
Project description:Hfq-dependent transcriptional alterations in the nitrogen-fixing endosymbiont S. meliloti. Comparison: S. meliloti 1021 strain Vs S. meliloti 1021Dhfq (containing a deletion of the hfq ORF).