Project description:S. aureus SA564 and SA564-codY-mutant were grown in bovine aqueous humor, bovine vitreous humor and a chemically defined medium. Samples were extracted in midlog phase and affymetrix microarray processing was performed.
Project description:Genomic DNA was collected from ies6d or ino80d after undergoing 40 or 80 generations post-sporulation of IES6/ies6 or 20 or 40 generations post-sporulation of INO80/ino80 heterozygous diploid strains, respectively. (1 day = 10 generations.) Genomic DNA was then amplified, and hybridized to a whole genome tiling microarray. Copy number variation was measured relative to a G1 arrested wild type sample.
Project description:Whole-genome tiling arrays were used to validate deletions and tandem duplications that were inferred based on next-generation sequencing data. The arrays were generated for six samples of the Drosophila melanogaster Genetic Reference Panel (DGRP) as well as the Berkeley reference strain. Structural variations (SVs) were assessed by comparing probe intensities within the region of interest between the sample for which the SV was predicted and the reference strain.