In budding yeast, as in other eukaryotes, the Cdc7 protein kinase is important for initiation of DNA synthesis in vegetative cells. In addition, Cdc7 has crucial meiotic functions: it facilitates premeiotic DNA replication, and it is essential for the initiation of recombination. This work uses a chemical genetic approach to demonstrate that Cdc7 kinase has additional roles in meiosis. First, Cdc7 allows expression of NDT80, a meiosis-specific transcriptional activator required for the induction ...[more]
Project description:3 replicates of WT and srbA knockout strains grown on GMM plates, harvested spores and inoculated 5x10^5 spores/ml in 300 ml of LGMM. Cultures incubated for 10h at 28C and then shifted for 2h to 37C (both at 200rpm) so they are just germinating at the start of the treatment. Cultures were shifted to hypoxia (37C, 200rpm) and incubated for 1hr. Samples were collected by pouring them over a filter paper in a Buechner funnel and snap froze the cells immediately in liquid nitrogen, and stored the cells at -80C and lyophilized all tissue at the same time after all samples were collected. RNA extraction was then done simultaneously with all time points.
Project description:Comparison of wild type and bir6 mutant Arabidopsis thaliana seedlings. The bir6 mutant is resistant to root growth inhibition by buthione sulfoxime, an inhibitor of glutathione biosynthesis.
Project description:In this study we explored the antiviral gene expression induced in the CNS of MHV-infected mice, by performing whole-genome expression profiling. Three different mouse strains (BALB/c, 129SvEv and 129SvEv IFNAR-/- mice), differing in their susceptibility to infection with MHV, were used.