Project description:In this study differences at the genetic level of 24 S. Enteritidis strains from six phage types were compared using Comparative Genomic Hybridisation (CGH) DNA microarrays to determine why some certain phage types are 'less successful' in causing human infection.
Project description:Genome-wide transcriptional profiling on microarray was performed for strain MC4100 in comparison to its otherwise isogenic ycgE::cat derivative. Strains were grown in LB at 28°C and cells were harvested at an OD (578nm) of 4.0.
Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.
Project description:Burkholderia cenocepacia J2315 was grown at 37 degrees centigrade in LB broth to the beginning of stationary phase (18 hours incubation time). <br>The expression profile was compared to cultures harvested in mid-log phase (7 hours incubation time).<br>
Project description:To investigate the effect for organisms by the treatment of the critical high remperature (CHT), we use Escherichia coli as a model organism and performed the transcriptome analysis to compare the genome wide transcriptional profiles between under the treatment of normal temperature, 37 degree C and 47 degree C.