The industrial use of elicitors as alternative tools for disease control needs the identification of abundant sources of them. We report on an elicitor obtained from the green algae Ulva spp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments on Medicago truncatula and compared to phytohormone effects. Ulvan induce ...[more]
Project description:We have previously showed a strong genetic determinant governing resistance of the M. truncatula A17 line to C. trifolii is located in a chromosomal region at the top of chromosome 4 (Ameline-torregrosa et al., 2008). This region also contains the RCT1 gene which has been shown to confer resistance to C. trifolii when transferred to a susceptible alfalfa plants (Zhu et al.), In order to evaluate the role of this region in the response to non-adapted Colletorichum species and to compare this response to those induce by C. trifolii, two M. truncatula near-isogenic lines differing only in this chromosomal region were used for transcript profiling experiments. These two lines were issued from a recombinant-inbred collection obtained from a A17-F83005.5 cross (Cazaux et al., in preparation). Plant inoculations were done on two-week-old entire plants by spraying a conidial suspension of C. trifolii or C. lindemuthianum to avoid possible artifacts which can be observed on detached leaves (Liu et al., 2007). RNA was extracted from leaves collected at 1dpi and 3dpi and transcript profiling was performed using Mt16kOLI1Plus chips (Thompson et al., 2005).
Project description:The objectif was to investigated the anti-atherogenic effects of nutritional supplementation of NAR in different mouse models of hypercholesterolemia and use transcriptomic approach to identify its molecular targets in the liver and aorta.
Project description:At eight weeks of age, male mice were divided into two groups (15 mice per group) fed iso-energetic diet: a control-diet or the same diet supplemented with 0.2% of curcumin (Sigma Aldrich) for 16 weeks (Table S1). The dose of curcumin used in this study is equivalent to 1g curcumin consumption in humans, a level of consumption achievable in South-Asian countries. No significant difference in weight gain was observed between the two groups at the end of the experimental period (data not shown). After 16 weeks, mice were anaesthetized (40 mg pentobarbital/kg of body weight) and blood was collected from the abdominal aorta into heparinised tubes and plasma was stored at -80 C. After washing with sterilized PBS, aorta and heart samples were immediately frozen in liquid nitrogen and stored at -80 C. Original processed data are available in the archive: http://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3185/E-MEXP-3185.additional.zip
Project description:ABA regulates in plants a wide range of developmental events, mediates responses to environmental stress and is necessary to proceed through seed maturation and to acquire desiccation tolerance and dormancy. Immuno-modulation is a suitable means to study ABA functions during seed maturation. Anti-ABA single chain antibody was expressed in pea seed driving LeB4-promoter (Saalbach et al., High-level expression of a single chain Fv fragment (scFv) antibody in transgenic pea seeds J. Plant Physiol. 2001 158: 529-533), which produced only a weak phenotype with slightly decreased seed weight, globulin/albumin and total nitrogen content (aABA line 16 cultivar Erbi). In another approach with a stronger, improved USP-promoter used to express the anti-ABA antibody in pea seeds a different phenotype emerged (aABA line 7, cultivar Eifel). In this line individual seed weight increased by 20 to 30% together with higher globulin and albumin content. To dissect the aABA phenotype at the molecular level, a search for genes with differential expression patterns in transgenic plant versus wild type seeds has been performed using 6k-oligo microarray analysis. cDNA probes were prepared from RNA isolated from embryo of developing seeds of wild type (12, 18, and 22 DAP) and transgenic aABA plants (12, 18, and 22 DAP), which correspond to the transition phase of seed development, and 6k-oligo microarray.
Project description:Medicago truncatula Tnt-1 insertional mutant for MtTTG1 gene (NF977) versus the wild-type R108. The seed samples are harvested at 16 days after pollination. Their transcript profiles were compared.