Transcriptomics

Dataset Information

3

Transcription profiling by array of human primary prostate epithelial and stromal cells after treatment with 4-methylsulphinylbutyl and 3-methylsulphinypropyl isothiocyanates


ABSTRACT: BACKGROUND: Broccoli consumption has been associated with a reduction in risk of prostate cancer. Isothiocyanates (ITC) derived from glucosinolates that accumulate in broccoli are potential dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) accumulates in heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) accumulates in sprouting broccoli. While, there are many studies regarding the biological activity of SF, there are few studies associated with IB. Moreover, the majority of studies have been undertaken with transformed cells, usually epithelial in origin, that have cancerous phenotypes.
METHOD: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to quantify global gene expression changes in these cells, following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by RT PCR.
RESULTS: Global gene expression profiling identified epithelial and stromal specific gene expression profiles. IB and SF induced different changes in gene expression in both epithelial and stromal cells but these changes were associated with similar functions
CONCLUSION: This data suggests that IB and SF both induce genes associated with cancer prevention, and IB should be further investigated as a potential chemo-preventative target.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

DISEASE(S): Benign Prostatic Hyperplasia,Prostate Carcinoma

SUBMITTER: Karen F Chambers  

PROVIDER: E-MEXP-2034 | ArrayExpress | 2009-06-05

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MEXP-2034.README.txt Txt
E-MEXP-2034.eSet.r Other
E-MEXP-2034.idf.txt Idf
E-MEXP-2034.processed.1.zip Processed
E-MEXP-2034.raw.1.zip Raw
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Publications

Gene expression profile of primary prostate epithelial and stromal cells in response to sulforaphane or iberin exposure.

Chambers Karen F KF   Bacon James R JR   Kemsley E Katherine EK   Mills Robert D RD   Ball Richard Y RY   Mithen Richard F RF   Traka Maria H MH  

The Prostate 20090901 13


<h4>Background</h4>Broccoli consumption has been associated with a reduced risk of prostate cancer. Isothiocyanates (ITCs) derived from glucosinolates that accumulate in broccoli are dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) derives from heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) from sprouting broccoli. While there are many studies regarding the biological activity of SF, mainly undertaken with cancerous  ...[more]

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