Transcriptomics

Dataset Information

7

Transcription profiling of Leishmania infantum, Leishmania major, Leishmania braziliensis intracellular amastigotes shows low level modulation between species and in different host genetic backgrounds


ABSTRACT: Genome sequencing of species of the kinetoplastid parasite, Leishmania, that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species studied to date, L. major, L. infantum and L. braziliensis. Here, we focus on RNA expression in the disease-promoting intracellular amastigotes and use customised oligonucleotide microarrays to confirm that all of these differentially-distributed genes are expressed in this critical stage of the parasite life cycle, with only a few regulated between species.

DISEASE(S): N/a (culture-derived),Cutaneous (footpad-derived),Visceral (spleen-derived),N/a (macrophage-derived)

SUBMITTER: Deborah F Smith  

PROVIDER: E-MEXP-2063 | ArrayExpress | 2009-04-01

REPOSITORIES: ArrayExpress

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Publications

Comparative expression profiling of Leishmania: modulation in gene expression between species and in different host genetic backgrounds.

Depledge Daniel P DP   Evans Krystal J KJ   Ivens Alasdair C AC   Aziz Naveed N   Maroof Asher A   Kaye Paul M PM   Smith Deborah F DF  

PLoS neglected tropical diseases 20090707 7


Genome sequencing of Leishmania species that give rise to a range of disease phenotypes in the host has revealed highly conserved gene content and synteny across the genus. Only a small number of genes are differentially distributed between the three species sequenced to date, L. major, L. infantum and L. braziliensis. It is not yet known how many of these genes are expressed in the disease-promoting intracellular amastigotes of these species or whether genes conserved between the species are di  ...[more]

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