The treatment groups were as follows (i) sham operation or LAD occlusion with infusion of (ii) saline, (iii) 15 ?g/kg Ucn I, (iv) 15 ?g/kg Ucn II and (v) 100 mg/kg tempo infused just prior to reperfusionl.
Following 2 hr reperfusion the left ventricle was removed, snap frozen, followed by RNA extraction.
INSTRUMENT(S): 418 [Affymetrix]
ORGANISM(S): Rattus norvegicus
DISEASE(S): Ischaemia Reperfusion Injury
SUBMITTER: Sean P Barry
Barry Seán P SP Ounzain Samir S McCormick James J Scarabelli Tiziano M TM Chen-Scarabelli Carol C Saravolatz Louis I I LII Faggian Giuseppe G Mazzucco Alessandro A Suzuki Hisanori H Thiemermann Christoph C Knight Richard A RA Latchman David S DS Stephanou Anastasis A
International journal of cardiology 20111024 3
BACKGROUND:IL-17A and IL-17F are pro-inflammatory cytokines which induce the expression of several cytokines, chemokines and matrix metalloproteinases (MMPs) in target cells. IL-17 cytokines have recently attracted huge interest due to their pathogenic role in diseases such as arthritis and inflammatory bowel disease although a role for IL-17 cytokines in myocardial infarction (MI) has not previously been described. METHODS:In vivo MI was performed by coronary artery occlusion in the absence or ...[more]