Name: Transcription profiling of canola hypercotyls and the outer inegument of the seed coat
Keywords: transcription profiling by array

OmicsDI

Browse
Submit Data
Databases
API
Help
Login

Dataset Information

12 Views

2 Connections

0 Citations

0 Reanalyses

7 Downloads

Omics score: 4

Transcription profiling of canola hypercotyls and the outer inegument of the seed coat

ABSTRACT: The transcriptome of outer integument of canola seed coat was compared to seven day old canola hypocotyls using the Brassica 90kCombimatrix microarray

ORGANISM(S): Brassica Napus

SUBMITTER: Yuanqing Jiang

PROVIDER: E-MEXP-2103 | ArrayExpress | 2010-09-01

REPOSITORIES: ArrayExpress

ACCESS DATA

Json Xml

Dataset's files

Source:

primary

			Action	DRS
	E-MEXP-2103.README.txt	Txt
	E-MEXP-2103.eSet.r	Other
	E-MEXP-2103.idf.txt	Idf
	E-MEXP-2103.idf.txt_original	Idf
	E-MEXP-2103.processed.1.zip	Processed

Items per page:

1 - 5 of 7

Publications

Transcriptome analysis of secondary-wall-enriched seed coat tissues of canola (Brassica napus L.).

Jiang Yuanqing Y Deyholos Michael K MK

Plant cell reports 20100210 4

The seed coat of Brassica napus (canola, oilseed rape) is derived from ovule integuments and contains a layer of palisade cells, which have thick secondary walls. Because cellulosic walls and other indigestible components of the seed coat contribute negatively to the value of oilseeds, efforts are underway to alter seed development. To facilitate these efforts, and to better understand the biology of seed coats, we used a 90,000 element microarray to identify genes whose transcripts were express ...[more]

PMID: 20145934

Similar Datasets

Transcription profiling by array of human post-radiotherapy-induced thyroid tumors and sporadic counterparts to identify a discriminatory gene expression signature

Project description:Identification of a stable gene expression signature with high classifying potential to discriminate post-radiotherapy-induced thyroid tumors (follicular adenomas and papillary carcinomas) from their sporadic counterparts.

2010-08-01 | E-MEXP-2739 | ArrayExpress

Transcription profiling of human thyroid follicular adenomas and thyroid papillary carcinomas to discriminate benign and malignant tumors

Project description:Identification of a stable gene expression signature with high classifying potential to discriminate benign (Follicular adenomas) and malignant (papillary carcinomas) thyroid tumors

2010-08-01 | E-MEXP-2335 | ArrayExpress

Transcription profiling of rat heart after treatment with PETN or NTG

Project description:Male Wistar rats (6 weeks, 250g), (Charles River, Sulzfeld, Germany) were equipped with micro osmotic pumps model 2001 from Alzet (Cupertino, CA) containing 400mM PETN dissolved in DMSO or the solvent and infusion was maintained for four days at 1?l/h (10.5?g/kg/min). For direct comparison, rats were also infused with 450mM NTG (6.6?g/kg/min in ethanol or the solvent as a control). <br>After 4d rats were sacrificed by exsanguinations under Isofluran anesthesia (5% inhalant in room air), the heart was rapidly excised, placed in ice-cold Krebs-HEPES-solution (composition in g/l: 5.78 NaCl, 0.35 KCl, 0.37 CaCl2, 0.30 MgSO4, 2.1 NaHCO3, 0.14 K2HPO4, 5.21 HEPES and 2.0 D-glucose) and dissected.<br>Total RNA was isolated from the hearts. Direct labeled cDNA was synthezised and hybridized to Rat OpArray Microarray (total genom expression profiling).

2010-02-06 | E-MEXP-2043 | ArrayExpress

Transcription profiling of white clover plants following infection by white clover mosaic virus

Project description:White clover mosaic virus (WCMV) is a major pathogen of white clover (Trifolium repens L.), with significant effects on yield and persistence. Due to the absence of natural sources of WCMV resistance a transgenic strategy has been employed to produce plants constitutively expressing WCMV replicase gene derivatives, designed to inhibit the propagation of WCMV through an RNA silencing mechanism. A 12,000 feature oligonucleotide microarray has been used to identify global changes in host plant, in addition to virus genome-encoded gene expression associated with WCMV infection in non-transgenic and transgenic WCMV-resistant white clover. Pairwise comparison between the transcriptome of mock-inoculated non-transgenic and WCMV-inoculated transgenic plants provides clear evidence for substantial equivalence between these two genotype/treatments, and demonstrate the efficacy of the transgenic strategy. WCMV- inoculated non-transgenic plants exhibit elevated abundance of many virus-encoded, and host immune response-specific transcripts compared to the transgenic resistant plants or mock-inoculated non-transgenic plants. By contrast, relative to inoculated sensitive plants, the majority of significantly up-regulated genes in mock-inoculated non-transgenic plants or WCMV-inoculated transgenic plants are markers of healthy cellular function. These results, and the occurrence of levels of WCMV-encoded transcripts in inoculated transgenic plants equivalent to those in virus-free plants, confirm the validity of the transgenic RNA silencing approach.<br>

2010-06-30 | E-MEXP-1688 | ArrayExpress

Transcription profiling by array of mouse paws from an in vivo model of collagen-induced arthritis to identify (s)-curvularin target genes

Project description:In previous studies, we identified the fungal macrocyclic lactone (S)-curvularin (SC) as an anti-inflammatory agent using a screening system detecting inhibitors of the Janus kinase/signal transducer and activator of transcription pathway. The objective of the present study was to investigate whether SC is able to decrease proinflammatory gene expression in an in vivo model of a chronic inflammatory disease. Therefore, the effects of SC and dexamethasone were compared in the model of collagen-induced arthritis (CIA) in mice. In addition to measuring the arthritis index (paw swelling) of the animals, we also performed whole genome microarray analyses to identify SC target genes and new therapeutic targets of SC.

2012-09-21 | E-MEXP-3180 | ArrayExpress

Expression of the Trehalose-6-phosphate Synthase 1 of Yeast Affects Gene Expression and Sugar Content in Potato Leaves

Project description:Effect of the TPS1 transgene on the transcriptome in potato

2011-08-17 | E-MEXP-3221 | ArrayExpress

Induced inflammation perturbs testis formation in zebrafish

Project description:Effect of heat-killed E. coli on zebrafish sex determination.

2012-06-08 | E-MEXP-3249 | ArrayExpress

Transcription profiling of Arabidopisis seedlings expressing E. coli gene otsA or otsB

Project description:comparison of effect of expressing E. coli gene otsA or otsB with wild type in 7-day-old seedlings grown in half MS

2009-03-20 | E-MEXP-2091 | ArrayExpress

Transcription profiling by array of potato leaves after drought stress

Project description:The effect of drought stress on wild-tpye and transgenic potato expressing the TPS1 gene of yeast was studied.