Transcription profiling of Candida albicans after induction of tet-OP4 promoter-EFG1 fusion under normoxia
ABSTRACT: Transformants either containing pCS1 (strain CCS1) or pNIM as control (strain CCS2) were induced by doxycycline (40 ﾵg/ml) for the indicated times (T0-T10, 0 to 10 min) in aerated hypha-inducing medium (10 % serum, 37 ﾰC). For each time point, cDNA of CCS1 and CCS2 were co-hybridised to microarrays and ratios of expression were calculated for each gene. Each gene is represented by duplicate spots on the DNA microarrays.
Project description:Transformants either containing pCS1 (strain CCS1) or pNIM as control (strain CCS2) were induced by doxycycline (40 ﾵg/ml) for the indicated times (T0-T10, 0 to 10 min) in hypha-inducing medium (10 % serum, 37 ﾰC) under a stream of 93.8 % nitrogen, 6 % CO2 and 0.2 % oxygen. For each time point, cDNA of CCS1 and CCS2 were co-hybridised to microarrays and ratios of expression were calculated for each gene. Each gene is represented by duplicate spots on the DNA microarrays.
Project description:This experiment evaluates quick (alarm) response to chilling in chilling-sensitive maize plants.<br>Maize inbred line cm109 were grown in optimal conditions until third leaf was fully developed. <br>At this stage plants were divided into three experimental variants: k0 - control plants, frozen<br>at the beginning of daylight, k4 - control plants kept in the same conditions and frozen after 4 hours<br>since beginning of daylight, c4 - plants kept in 14 deg. C for 4 hours since "dawn". At the mentioned<br>moments, leaves were harvested and frozen in liquid nitrogen for RNA isolation.
Project description:<br><br>Annual heart allograft failure in humans rates about 3-5%. The main reason after the first postoperative year is chronic rejection. Myointimal hyperplasia, the hellmark of chronic rejection, results in a specific type of ischemic heart disease. The lack of angina pectoris symptoms allow ventricular arrythmias, sudden cardiac death or heart failure to occur without warning. In addition, diagnostic tools such as endomyocardial biopsy, coronary angiography or intracoronary ultrasound fail to predict the individual risk for myocardial dysfunction.<br><br>The mechanisms responsible for chronic rejection are predominantly alloimmune mediated with activated T cells, macrophages, B cell mediated antibody formation and secreted cytokines responding to HLA and other endothelial cell antigens. In addition, non immunologic risk factors such as recipient age, metabolic factors, hypertension and ischemia contribute to development of this disease. Previous studies have demonstrated that ischemia has a profound influence on short term allograft survival but the underlaying mechanisms remain largely unknown. Apoptosis seems to play a crucial role in ischemia/reperfusion injury and several mechanisms for programmed cell death have been described. However, consequences on long term cell function of viability have not been investigated. <br><br>The aim of this study was to investigate the implication and the mechanism of prolonged cold organ storage as a non immunologic risk factor in the pathogenesis of chronic rejection in a cardiac allograft model. <br><br>We aimed for answering the following specific questions:<br><br>How does cold ischemia affect the alloimmue response short and long term? <br><br>How does prolonged cold ischemia affect gene expression at later time points after transplantation? <br><br>Does it influence gene expression during chronic rejection?<br><br><br><br>
Project description:Comparison of wild type and bir6 mutant Arabidopsis thaliana seedlings. The bir6 mutant is resistant to root growth inhibition by buthione sulfoxime, an inhibitor of glutathione biosynthesis.
Project description:Male C57B1/CBA mice were exposed to mainstream tobacco smoke (MTS) from two cigarettes daily, 5 days/week for 6 or 12 weeks. Mice were sacrificed immediately, or six weeks following, the last cigarette. High density DNA microarrays were used to characterize global gene expression changes in whole heart.<br><br>Western blot and ELISA assays were used to determine levels of Cyp1A1 protein in heart microsomes and total and active PAI-1 protein from tissue extracts.
Project description:Transcriptional profiling of estrogen regulated genes in human primary osteoblasts. The cells were either treated with estradiol (1 nM) or the pure antagonist ICI 182,780 (Faslodex), which acts as a potent inhibitor of estrogen receptor signaling.
Project description:Exponentially growing Sulfolobus acidocaldarius were treated with NaAc to generate replication runout and arrest in G2 phase. The cells were then resuspended in fresh acetate-free media which generates a synchronous population. Samples for investigation of gene expression change were taken during the synchronised populations progress through the cell cycle.