Transcriptomics

Dataset Information

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Transcription profiling by array of human MOLT4 cells after treatment with actinomycin D


ABSTRACT: MOLT4 cells in log phase (1x106 cells/ml) were irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a 137Cs ?-irradiator. After 4 hours, actinomycin D (5 ?M) was added to block transcription. Aliquots of culture were removed at hourly intervals and RNA was extracted (Trizol; Invitrogen). RNA and cRNA quantity and quality was determined by Nanodrop spectrophotometer and Bioanalyser 2100 (Agilent). Transcript levels were determined at different time points using Affymetrix Human U133A microarrays.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Efterpi Papouli  

PROVIDER: E-MEXP-2179 | ArrayExpress | 2009-06-30

REPOSITORIES: ArrayExpress

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