Transcriptomics

Dataset Information

2

Transcription profiling of Brugia Wolbachia elimination


ABSTRACT: Female worms (Brugia malayi) were collected from infected jirds treated with 2.5 mg/ml tetracycline in drinking water for 7, 14, or 21 days to eliminate the worm's endosymbiont, Wolbachia.
Control age matched female worms were recovered from infected jirds given normal water for drinking.
The Filarial Nematode Oligonucleotide Array (version 2) was used in hybridization analyses on cDNA generated from extracted total RNA.
Each microarray was hybridized with a mixture of control and experimental cDNA differentially labeled with Cy3 and Cy5 in a flip-dye experiment.
Gridding and analysis of images were performed using ScanArray v3.0, each spot defined pixel-by-pixel using a modified Mann-Whitney test, and the resulting values processed with Gene-Spring 7.1 software.

ORGANISM(S): Brugia malayi  

SUBMITTER: Jay DePasse  

PROVIDER: E-MEXP-2185 | ArrayExpress | 2009-05-22

REPOSITORIES: ArrayExpress

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Publications

Brugia malayi gene expression in response to the targeting of the Wolbachia endosymbiont by tetracycline treatment.

Ghedin Elodie E   Hailemariam Tiruneh T   DePasse Jay V JV   Zhang Xu X   Oksov Yelena Y   Unnasch Thomas R TR   Lustigman Sara S  

PLoS neglected tropical diseases 20091006 10


Brugia malayi, like most human filarial parasite species, harbors an endosymbiotic bacterium of the genus Wolbachia. Elimination of the endosymbiont leads to sterilization of the adult female. Previous biochemical and genetic studies have established that communication with its endobacterium is essential for survival of the worm.We used electron microscopy to examine the effects of antibiotic treatment on Wolbachia cell structure. We have also used microarray and quantitative RT-PCR analyses to  ...[more]

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