Dataset Information


Transcription profiling by array of rice grown in different light and temperature cycles

ABSTRACT: Rice (Oryza sativa, ssp. Japonica, cv. Nipponbare 1) plants were grown in a Conviron PGR 15 growth chamber using precise control of temperature, light, and humidity.
Diurnal (driven) conditions included 12L:12D light cycles and 31C/20C thermocycles in three different combinations. These were: photocycles (LDHH), 12 hrs. light (L)/12 hrs. dark (D) at a constant temperature (31C; HH); photo/thermocycles (LDHC): 12 hrs. light (L) /12 hrs. dark (D) with a high day temperature (31C) and a low night temperature (20C); and thermocycles (LLHC): continuous light (LL) with 12 hrs. high/12 hrs. low temperature (31C, day; 20C, night). Light intensity and relative humidity were 1000 micromol m-2s-2 and 60%, respectively.
Three-month-old rice plants were entrained for at least one week under the respective condition prior to initiation of each experiment. Leaves and stems from individual rice plants were collected every four hours for 48 hrs in driven (diurnal) conditions followed by a two day freerun spacer under continuous light/temperature followed by two additional days of sampling under the same continuous free run condition.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Oryza sativa  

SUBMITTER: Sergei Filichkin  

PROVIDER: E-MEXP-2506 | ArrayExpress | 2010-01-09


Dataset's files

Action DRS
E-MEXP-2506.README.txt Txt
E-MEXP-2506.eSet.r Other
E-MEXP-2506.idf.txt Idf Processed Raw
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Global profiling of rice and poplar transcriptomes highlights key conserved circadian-controlled pathways and cis-regulatory modules.

Filichkin Sergei A SA   Breton Ghislain G   Priest Henry D HD   Dharmawardhana Palitha P   Jaiswal Pankaj P   Fox Samuel E SE   Michael Todd P TP   Chory Joanne J   Kay Steve A SA   Mockler Todd C TC  

PloS one 20110609 6

<h4>Background</h4>Circadian clocks provide an adaptive advantage through anticipation of daily and seasonal environmental changes. In plants, the central clock oscillator is regulated by several interlocking feedback loops. It was shown that a substantial proportion of the Arabidopsis genome cycles with phases of peak expression covering the entire day. Synchronized transcriptome cycling is driven through an extensive network of diurnal and clock-regulated transcription factors and their target  ...[more]

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