Transcription profiling by array of Arabidopsis grown in either a light/dark cycle or continuous light
ABSTRACT: Transcript profiling was performed on samples derived from plants grown under long day conditions. Time series were harvested under diurnal (L/D; light/dark cycles) at control temperature (20 C) and cold (4 C); and under circadian conditions (L/L; continuous light) at control temperature (20 C). Leaves were sampled at time 0 and after 2 h (to coincide with light-dark transitions) and then every 4 h until 58 h.
In plants, there is a large overlap between cold and circadian regulated genes and in Arabidopsis, we have shown that cold (4°C) affects the expression of clock oscillator genes. However, a broader insight into the significance of diurnal and/or circadian regulation of cold responses, particularly for metabolic pathways, and their physiological relevance is lacking. Here, we performed an integrated analysis of transcripts and primary metabolites using microarrays and gas chromatography-mass spec ...[more]
Project description:The aim of this experiment is to test the ability of the ortholog of Arabidopsis LFY gene from flowering and non flowering species to complement an Arabidopsis LFY mutant. <br>Plants expressing, in a homozygous lfy12 background, the open reading frame of LFY orthologs under the control of Arabidopsis LFY promoter were synchronously induced to flower by growing plants in short days for 30 days then shifting them to Long Day for an additional 8 days. Shoot apices were dissected at either d0 or d8 in long days. Two biological replicates were performed. The following genotypes were used: <br>Col - wild type arabidopsis; reference strain<br>LFY (lfy12; LFY::AthLFY) - Arabidopsis<br>UNI (lfy12; LFY::UNI) - Pisum<br>ALF (lfy12; LFY::ALF) - Petunia <br>WelNDLY (lfy12; LFY::WelNDLY) - Welwitschia mirabilis<br>CrLFY1 (lfy12; LFY::CrLFY1) - Ceratopteris richardii<br>CrLFY2 (lfy12; LFY::CrLFY2) - Ceratopteris richardii<br>lfy-2; weak lfy allele<br>lfy-9; intermediate leafy allele<br>lfy-12; strong leafy allele.
Project description:In gain of function studies, phloem intercalated with xylem (PXY) signalling has previously been shown to promote vascular cell division. However, no reduction in vascular cell division has been reported in pxy mutants. This suggests that a compensatory mechanism promotes vascular cell division in the absence of PXY. To uncover this mechanism, the transcriptome of pxy mutants was compared to wild type counterparts.
Project description:White clover mosaic virus (WCMV) is a major pathogen of white clover (Trifolium repens L.), with significant effects on yield and persistence. Due to the absence of natural sources of WCMV resistance a transgenic strategy has been employed to produce plants constitutively expressing WCMV replicase gene derivatives, designed to inhibit the propagation of WCMV through an RNA silencing mechanism. A 12,000 feature oligonucleotide microarray has been used to identify global changes in host plant, in addition to virus genome-encoded gene expression associated with WCMV infection in non-transgenic and transgenic WCMV-resistant white clover. Pairwise comparison between the transcriptome of mock-inoculated non-transgenic and WCMV-inoculated transgenic plants provides clear evidence for substantial equivalence between these two genotype/treatments, and demonstrate the efficacy of the transgenic strategy. WCMV- inoculated non-transgenic plants exhibit elevated abundance of many virus-encoded, and host immune response-specific transcripts compared to the transgenic resistant plants or mock-inoculated non-transgenic plants. By contrast, relative to inoculated sensitive plants, the majority of significantly up-regulated genes in mock-inoculated non-transgenic plants or WCMV-inoculated transgenic plants are markers of healthy cellular function. These results, and the occurrence of levels of WCMV-encoded transcripts in inoculated transgenic plants equivalent to those in virus-free plants, confirm the validity of the transgenic RNA silencing approach.<br>
Project description:Transcriptional Regulation in liver and ovary associated with developmental and reproductive effects in female Zebrafish exposed to natural mixtures of persistent organic pollutants from Lake Mjosa or Lake Losna