Transcriptomics

Dataset Information

6

Transcription profiling by array of Arabidopsis grown in either a light/dark cycle or continuous light


ABSTRACT: Transcript profiling was performed on samples derived from plants grown under long day conditions. Time series were harvested under diurnal (L/D; light/dark cycles) at control temperature (20 C) and cold (4 C); and under circadian conditions (L/L; continuous light) at control temperature (20 C). Leaves were sampled at time 0 and after 2 h (to coincide with light-dark transitions) and then every 4 h until 58 h.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Carmen Espinoza  

PROVIDER: E-MEXP-2526 | ArrayExpress | 2010-12-20

REPOSITORIES: ArrayExpress

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Publications

Interaction with diurnal and circadian regulation results in dynamic metabolic and transcriptional changes during cold acclimation in Arabidopsis.

Espinoza Carmen C   Degenkolbe Thomas T   Caldana Camila C   Zuther Ellen E   Leisse Andrea A   Willmitzer Lothar L   Hincha Dirk K DK   Hannah Matthew A MA  

PloS one 20101123 11


In plants, there is a large overlap between cold and circadian regulated genes and in Arabidopsis, we have shown that cold (4°C) affects the expression of clock oscillator genes. However, a broader insight into the significance of diurnal and/or circadian regulation of cold responses, particularly for metabolic pathways, and their physiological relevance is lacking. Here, we performed an integrated analysis of transcripts and primary metabolites using microarrays and gas chromatography-mass spec  ...[more]

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