Dataset Information


Transcription profiling of human plasmacytoid dentritic cells co-cultured with activated conventional dendritic cells

ABSTRACT: Two well-characterized blood dendritic cell populations, conventional (cDC) and plasmacytoid (pDC), exhibit multiple phenotypic, migratory and functional differences that suggest specialized and may be complementary and coordinated functions. To study this possible coordination, cDCs and pDCs from healthy blood donors were sorted and cDCs were stimulated either with LPS or R848 whereas pDCs were CFSE-labelled and maintained in IL3. Then, CFSE labelled-pDCs and stimulated-cDCs were mixed and co-cultured. Following this "conditioning", CFSE-pDCs were sorted again and further analyzed. Conditioned pDCs showed moderate phenotypic maturation and acquired allostimulatory capacity. Microarray and RT-PCR analyses showed the induction of different genes including chemokines and proinflammatory cytokines that were dependent on the stimulation received from the "conditioner" cDC. Additionally, the differential pattern of conditioning was confirmed by protein secretion analyses with the production of specific chemokines and cytokines by conditioned pDCs. Importantly, conditioning of pDCs by activated cDCs required cell-cell contact.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo Sapiens

SUBMITTER: Francesc E Borras  

PROVIDER: E-MEXP-2555 | ArrayExpress | 2011-01-20


Dataset's files

Action DRS
E-MEXP-2555.README.txt Txt
E-MEXP-2555.eSet.r Other
E-MEXP-2555.idf.txt Idf
E-MEXP-2555.idf.txt_original Idf Processed
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TLR-activated conventional DCs promote γ-secretase-mediated conditioning of plasmacytoid DCs.

Pérez-Cabezas Begoña B   Naranjo-Gómez Mar M   Ruiz-Riol Marta M   Bastos-Amador Patricia P   Fernández Marco A MA   Carmona Francesc F   Nuñez Fatima F   Pujol-Borrell Ricardo R   Borràs Francesc E FE  

Journal of leukocyte biology 20120424 1

Cooperative events between DC subsets involve cell contact and soluble factors. Upon viral challenge, murine pDCs induce cDC cooperation through CD40-CD40L interactions and IL-15 secretion, whereas in humans, the same effect is mediated by IFN-α. Conversely, during bacterial infections, pDC maturation may be induced by activated cDCs, although no mechanisms had been described so far. Here, we investigate how human pDCs are "conditioned" by cDCs. Blood-borne DC subsets (cDCs and pDCs) were sorted  ...[more]

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