Dataset Information


Transcription profiling by array of Ralsonia eutropha H16 wild type and glucose-utilizing mutant G+1 cultivated in medium with fructose or glucose

ABSTRACT: In order to identify genes which contribute on the uptake of glucose into cells of the mutant R. eutropha G+1, a genome wide transcription analyses was done. Transcripts of strain H16 and the glucose-utilizing mutant R. eutropha G+1, cultivated in mineral salts medium supplemented with either fructose or glucose were compared.

ORGANISM(S): Cupriavidus necator H16  

SUBMITTER: Katja Peplinski  

PROVIDER: E-MEXP-2662 | ArrayExpress | 2011-04-01


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Proteomic and transcriptomic elucidation of the mutant ralstonia eutropha G+1 with regard to glucose utilization.

Raberg Matthias M   Peplinski Katja K   Heiss Silvia S   Ehrenreich Armin A   Voigt Birgit B   Döring Christina C   Bömeke Mechthild M   Hecker Michael M   Steinbüchel Alexander A  

Applied and environmental microbiology 20110128 6

By taking advantage of the available genome sequence of Ralstonia eutropha H16, glucose uptake in the UV-generated glucose-utilizing mutant R. eutropha G(+)1 was investigated by transcriptomic and proteomic analyses. Data revealed clear evidence that glucose is transported by a usually N-acetylglucosamine-specific phosphotransferase system (PTS)-type transport system, which in this mutant is probably overexpressed due to a derepression of the encoding nag operon by an identified insertion mutati  ...[more]

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